Naive Human Embryonic Stem Cells Can Give Rise to Cells with a Trophoblast-like Transcriptome and Methylome
| العنوان: | Naive Human Embryonic Stem Cells Can Give Rise to Cells with a Trophoblast-like Transcriptome and Methylome |
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| المؤلفون: | Takahiro Arima, Hsin Wei Tseng, Wanlu Liu, Jessica K Cinkornpumin, Yixin Guo, William A. Pastor, Sin Young Kwon, Thomas F. Duchaine, Jacinthe Sirois, Ishtiaque Hossain, Colleen S. Russett, Hiroaki Okae |
| المصدر: | Stem Cell Reports |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, placenta, Somatic cell, Human Embryonic Stem Cells, Integrin alpha2, Syncytiotrophoblasts, Biology, Biochemistry, Article, Transcriptome, 03 medical and health sciences, Genomic Imprinting, 0302 clinical medicine, Pregnancy, Placenta, Genetics, medicine, Humans, Blastocyst, development, reproductive and urinary physiology, amnion, DNA methylation, epigenetics, Trophoblast, Reproducibility of Results, Cell Biology, differentiation, embryonic stem cells, Epithelial Cell Adhesion Molecule, pluripotency, Embryonic stem cell, trophoblast, 3. Good health, Cell biology, Trophoblasts, Pregnancy Trimester, First, 030104 developmental biology, medicine.anatomical_structure, embryonic structures, Cell Transdifferentiation, Female, Stem cell, 030217 neurology & neurosurgery, Developmental Biology |
| الوصف: | Summary Human embryonic stem cells (hESCs) readily differentiate to somatic or germ lineages but have impaired ability to form extra-embryonic lineages such as placenta or yolk sac. Here, we demonstrate that naive hESCs can be converted into cells that exhibit the cellular and molecular phenotypes of human trophoblast stem cells (hTSCs) derived from human placenta or blastocyst. The resulting “transdifferentiated” hTSCs show reactivation of core placental genes, acquisition of a placenta-like methylome, and the ability to differentiate to extravillous trophoblasts and syncytiotrophoblasts. Modest differences are observed between transdifferentiated and placental hTSCs, most notably in the expression of certain imprinted loci. These results suggest that naive hESCs can differentiate to extra-embryonic lineage and demonstrate a new way of modeling human trophoblast specification and placental methylome establishment. Graphical Abstract Highlights • Naive hESCs can be transdifferentiated to hTSC-like cells and purified by FACS • Transdifferentiated hTSCs (tdhTSCs) are similar to hTSCs from placenta • tdhTSCs have placenta-like methylome • Modest methylation abnormalities are observed in tdhTSCs Cinkornpumin and colleagues provide a protocol for converting naive human embryonic stem cells (hESCs) into transdifferentiated trophoblast stem cells (tdhTSCs). The resulting tdhTSCs show gene expression and differentiation capabilities akin to hTSCs from placenta or blastocyst. They also have a placenta-like methylome, although there are residual traces of hESC-methylation pattern. |
| تدمد: | 2213-6711 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba993b9044966e40c8f31e815a00a5ba https://pubmed.ncbi.nlm.nih.gov/32619492 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ba993b9044966e40c8f31e815a00a5ba |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22136711 |
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