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Characterization of a transgenic mouse model of chronic conditional platelet depletion

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العنوان: Characterization of a transgenic mouse model of chronic conditional platelet depletion
المؤلفون: Leah M. Wuescher, Sharmeen Nishat, Randall G. Worth
المصدر: Research and Practice in Thrombosis and Haemostasis
Research and Practice in Thrombosis and Haemostasis, Vol 3, Iss 4, Pp 704-712 (2019)
بيانات النشر: John Wiley and Sons Inc., 2019.
سنة النشر: 2019
مصطلحات موضوعية: Genetically modified mouse, Diphtheria toxin, Original Articles: Haemostasis, 0303 health sciences, Adoptive cell transfer, adoptive transfer, mice, lcsh:RC633-647.5, Chemistry, blood platelets, thrombocytopenia, lcsh:Diseases of the blood and blood-forming organs, Hematology, 030204 cardiovascular system & hematology, Pharmacology, Neuroprotection, 03 medical and health sciences, 0302 clinical medicine, Immunity, Hemostasis, Systemic administration, diphtheria toxin, Platelet, Original Article, 030304 developmental biology
الوصف: Background Platelets are widely recognized for their role in maintaining hemostasis. Recently, platelets have become appreciated for their varying roles in immunity, neuroprotection, and other physiological processes. While there are currently excellent methods to transiently deplete platelets and models of thrombocytopenia, studying the roles of platelets in chronic processes can be challenging. Objective Phenotypic characterization of the PF4‐DTR mouse model of conditional platelet depletion compared to antibody depletion. Methods We describe the ability of the PF4‐DTR mouse to maintain chronic platelet depletion, along with examining the bleeding phenotype compared to antibody‐mediated platelet depletion. Results Systemic administration of diphtheria toxin resulted in >99% platelet depletion that can be maintained for >2 weeks. When compared to an antibody depletion model, PF4‐DTR mice showed similar phenotypes when challenged with tail bleed and saphenous vein measurements of hemostasis. Mice depleted with diphtheria toxin were also able to undergo adoptive transfer of platelets. If the frequency and amount of diphtheria toxin is reduced, mice can be maintained at >40% depletion for >28 days, showing that this model is tunable. Conclusions When compared to the gold standard of antibody‐mediated depletion, PF4‐DTR mice showed similar phenotypes and should be considered an important tool for examining the impact of thrombocytopenia over longer periods of time.
اللغة: English
تدمد: 2475-0379
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::baeb25ebdc27ced78326dd7ea952b64f
http://europepmc.org/articles/PMC6781920
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....baeb25ebdc27ced78326dd7ea952b64f
قاعدة البيانات: OpenAIRE
الوصف
تدمد:24750379