Clinical application of cell-free next-generation sequencing for infectious diseases at a tertiary children’s hospital
| العنوان: | Clinical application of cell-free next-generation sequencing for infectious diseases at a tertiary children’s hospital |
|---|---|
| المؤلفون: | Helen Harvey, Jennifer Foley, Christopher R. Cannavino, Lauge Farnaes, Nicole G. Coufal, Julianne Wilke, Nanda Ramchandar, Leidy Tovar Padua, Adriana H. Tremoulet, Alice Pong |
| المصدر: | BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-6 (2021) BMC Infectious Diseases BMC infectious diseases, vol 21, iss 1 |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Future studies, Cell free, Infectious and parasitic diseases, RC109-216, Medical microbiology, Child, Children, Pediatric, screening and diagnosis, Cell-free plasma, High-Throughput Nucleotide Sequencing, Hospitals, Pediatric, Hospitals, Detection, Infectious Diseases, Medical Microbiology, Child, Preschool, Infectious etiology, Female, Infection, Cell-Free Nucleic Acids, 4.2 Evaluation of markers and technologies, medicine.medical_specialty, Adolescent, 030106 microbiology, Clinical Sciences, Microbiology, Communicable Diseases, 03 medical and health sciences, Diagnostic Tests, Clinical Research, Chart review, medicine, Humans, Routine, Intensive care medicine, Preschool, Reference standards, Retrospective Studies, business.industry, Diagnostic Tests, Routine, Research, Peripheral blood, 4.1 Discovery and preclinical testing of markers and technologies, 030104 developmental biology, Good Health and Well Being, Next-generation sequencing, Metagenome, Metagenomics, business, Pediatric population |
| الوصف: | Background Children affected by infectious diseases may not always have a detectable infectious etiology. Diagnostic uncertainty can lead to prolonged hospitalizations, inappropriately broad or extended courses of antibiotics, invasive diagnostic procedures, and difficulty predicting the clinical course and outcome. Cell-free plasma next-generation sequencing (cfNGS) can identify viral, bacterial, and fungal infections by detecting pathogen DNA in peripheral blood. This testing modality offers the ability to test for many organisms at once in a shotgun metagenomic approach with a rapid turnaround time. We sought to compare the results of cfNGS to conventional diagnostic test results and describe the impact of cfNGS on clinical care in a diverse pediatric population at a large academic children’s hospital. Methods We performed a retrospective chart review of hospitalized subjects at a tertiary pediatric hospital to determine the diagnostic yield of cfNGS and its impact on clinical care. Results We describe the clinical application of results from 142 cfNGS tests in the management of 110 subjects over an 8-month study period. In comparison to conventional testing as a reference standard, cfNGS was found to have a positive percent agreement of 89.6% and negative percent agreement of 52.3%. Furthermore, 32.4% of cfNGS results were directly applied to make a clinical change in management. Conclusions We demonstrate the clinically utility of cfNGS in the management of acutely ill children. Future studies, both retrospective and prospective, are needed to clarify the optimal indications for testing. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1471-2334 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb1e0c6a9b428e720b2f198b1642a2df https://doaj.org/article/afbffd9aefa045cc9d8a70209d9e3c1d |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bb1e0c6a9b428e720b2f198b1642a2df |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14712334 |
|---|