Effect of CCL11 on In Vitro Myogenesis and Its Clinical Relevance for Sarcopenia in Older Adults
| العنوان: | Effect of CCL11 on In Vitro Myogenesis and Its Clinical Relevance for Sarcopenia in Older Adults |
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| المؤلفون: | So Jeong Park, Jeoung Hee Kim, Da Ae Kim, Hee-Won Jung, Il-Young Jang, Seungjoo Lee, Jin Hoon Park, Beom-Jun Kim, Jin Young Lee, Eunju Lee |
| المصدر: | Endocrinology and Metabolism Endocrinology and Metabolism, Vol 36, Iss 2, Pp 455-465 (2021) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Chemokine CCL11, medicine.medical_specialty, CCR2, Sarcopenia, Aging, Endocrinology, Diabetes and Metabolism, 030209 endocrinology & metabolism, Muscle Development, Diseases of the endocrine glands. Clinical endocrinology, 03 medical and health sciences, Chemokine receptor, Mice, 0302 clinical medicine, Endocrinology, Internal medicine, medicine, Myocyte, Animals, Humans, Endocrine Research, Muscle Strength, Muscle, Skeletal, CCL11, Aged, Hand Strength, business.industry, Myogenesis, Skeletal muscle, respiratory system, RC648-665, medicine.disease, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Biomarker (medicine), Original Article, business, Biomarkers |
| الوصف: | Background: The C-C motif chemokine ligand 11 (CCL11) has been receiving attention as a potential pro-aging factor. Accordingly, it may be involved in muscle metabolism and sarcopenia, a key component of aging phenotypes. To clarify this potential, we investigated the effects of CCL11 on in vitro muscle biology and its clinical relevance for sarcopenia parameters in older adults.Methods: Myogenesis was induced in mouse C2C12 myoblasts with 2% horse serum. Human blood samples were collected from 79 participants who underwent a functional assessment. Thereafter, CCL11 level was measured using a quantikine ELISA kit. Sarcopenia was defined using the Asian-specific guideline.Results: Recombinant CCL11 treatment significantly stimulated myogenesis in a dose-dependent manner, and consistently increased the expression of myogenic differentiation markers. Among the C-C chemokine receptors (CCRs), CCR5, not CCR2 and CCR3, was predominantly expressed in muscle cells. Further, the CCR5 inhibitor blocked recombinant CCL11-stimulated myogenesis. In a clinical study, serum CCL11 level was not significantly different according to the status of sarcopenia, low muscle mass, weak muscle strength, and poor physical performance, and was not associated with skeletal muscle index, grip strength, short physical performance battery score, gait speed, and time to complete 5 chair stands, after adjusting for sex, age, and body mass index.Conclusion: Contrary to expectations, CCL11 exerted beneficial effects on muscle metabolism at least in vitro system. However, its impact on human muscle health was not evident, suggesting that circulating CCL11 may not be a useful biomarker for sarcopenia risk assessment in older adults. |
| تدمد: | 2093-5978 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb27f6b47c7cbbfc73e86ad3ff420620 https://pubmed.ncbi.nlm.nih.gov/33849248 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bb27f6b47c7cbbfc73e86ad3ff420620 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20935978 |
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