Characterization of Pectobacterium brasiliense strains from potato and vegetables in Israel
| العنوان: | Characterization of Pectobacterium brasiliense strains from potato and vegetables in Israel |
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| المؤلفون: | Valérie Hélias, S. Mordechai‐Lebiush, Marina Hazanovsky, Laura Chalupowicz, Shulamit Manulis-Sasson, Lea Valinsky, Angélique Laurent, Orly Erlich, O. Dror, M. Reuven, Leah Tsror |
| المساهمون: | Agricultural Research Organisation (ARO), Volcani Center, Fédération Nationale des Producteurs de Plants de Pomme de Terre (FN3PT), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Ministry of Health Tel Aviv, Ministry of Health |
| المصدر: | Plant Pathology Plant Pathology, 2021, 70 (9), pp.2179-2187. ⟨10.1111/ppa.13454⟩ |
| بيانات النشر: | HAL CCSD, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Pectobacterium, Blackleg, dnaX phylogeny, soft rot, blackleg, Plant Science, PFGE, Horticulture, Biology, Solanum tuberosum, biology.organism_classification, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, Botany, Genetics, Pulsed-field gel electrophoresis, Agronomy and Crop Science |
| الوصف: | International audience; Pectobacterium brasiliense (Pbr) infects a wide range of crops worldwide, causing potato blackleg and soft rot and vegetable soft rots. This study aimed to characterize the genetic diversity and virulence variability among 68 Pbr strains isolated from either symptomless potato progeny tubers, diseased potato plants, ware potatoes wash water, or vegetables grown in Israel, as well as strains isolated from symptomless seed tubers grown in Europe, or diseased potato plants grown in France. The collection was typed using PCR and TaqMan real-time PCR analyses, dnaX sequence analysis, pulsed-field gel electrophoresis (PFGE), and pectolytic activity. dnaX phylogeny grouped almost all strains in a common genetic clade related to Pbr, which was distinct from the other Pectobacterium species. PFGE analysis identified two main clusters, including one major group of 47 strains with 95%-100% similarity. Maceration assays on two potato cultivars showed significant differences between strains but with no correlations with the source of the strains nor the status of the host (with/without symptoms). Molecular (dnaX sequences and PFGE profiles) and phenotypic analyses (tuber maceration tests) showed that the tested Pbr strains are not a homogeneous group. Analysis of the tested Pbr strains isolated from potato and vegetables grown in fields with a history of potato cultivation suggests that seed tubers imported from Europe may be the main source for Pbr in Israel. To the best of our knowledge, this is the first study that describes biodiversity and population structure of P. brasiliense isolated from potato and vegetables under hot climate conditions |
| اللغة: | English |
| تدمد: | 0032-0862 1365-3059 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb3d401295c5a074bc26dba2a464085f https://hal.inrae.fr/hal-03738855 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....bb3d401295c5a074bc26dba2a464085f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00320862 13653059 |
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