False positive legionella serology in campylobacter infection: campylobacter serotypes, duration of antibody response and elimination of cross-reactions in the indirect fluorescent antibody test
| العنوان: | False positive legionella serology in campylobacter infection: campylobacter serotypes, duration of antibody response and elimination of cross-reactions in the indirect fluorescent antibody test |
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| المؤلفون: | G. Kudesia, T. C. J. Boswell, L. E. Marshall |
| المصدر: | Epidemiology and Infection. 112:347-357 |
| بيانات النشر: | Cambridge University Press (CUP), 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Adult, Male, Serotype, Time Factors, Adolescent, Epidemiology, Legionella, Fluorescent Antibody Technique, Cross Reactions, Immunofluorescence, medicine.disease_cause, Legionella pneumophila, Microbiology, Serology, Campylobacter Gastroenteritis, Campylobacter Infections, medicine, Humans, False Positive Reactions, Serotyping, Direct fluorescent antibody, medicine.diagnostic_test, biology, Campylobacter, biology.organism_classification, Antibodies, Bacterial, Virology, Gastroenteritis, Infectious Diseases, England, biology.protein, Female, Antibody, Research Article |
| الوصف: | SUMMARYSera from 83 patients with campylobacter gastroenteritis were examined for the presence of legionella antibodies by indirect immunofluorescence. Twenty–one patients (25%) had positive titres (≥ 16) including 11 patients with titres of ≥ 128. Legionella seropositivity persisted in 5 of 9 patients (55%) studied for 6–9 months. Campylobacter isolates were serotyped by the Penner scheme. Isolates associated with legionella seropositivity included Penner types 1, 2 and 4, the common endemic serotypes in England. Campylobacter blocking fluids were prepared from a range of Penner reference strains. The blocking fluid prepared from Penner type 11 was the most efficient at inhibiting the false-positive legionella titres. Using this absorption step legionella titres were inhibited from 24 of 26 patients (92%) with campylobacter but not from 8 patients with cultureproven legionnaires' disease. We recommend that this method is incorporated into routine diagnostic legionella serology in order to eliminate false-positive reactions due to campylobacter. |
| تدمد: | 1469-4409 0950-2688 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb4794c20fafcdc69cf51042a2d364e1 https://doi.org/10.1017/s0950268800057757 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bb4794c20fafcdc69cf51042a2d364e1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14694409 09502688 |
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