Dysferlin-deficient Muscular Dystrophy: Gadofluorine M Suitability at MR Imaging in a Mouse Model
| العنوان: | Dysferlin-deficient Muscular Dystrophy: Gadofluorine M Suitability at MR Imaging in a Mouse Model |
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| المؤلفون: | Semjon Taubert, Lutz Luedemann, Volkmar Gross, Antje Vieweger, Michael Obst, Bernd Misselwitz, Susanne Mueller, Matthias Gutberlet, Saskia Schmidt, Jens Steinbrink, Simone Spuler |
| المصدر: | Radiology. 250:87-94 |
| بيانات النشر: | Radiological Society of North America (RSNA), 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Contrast Media, Gadolinium, Mice, Inbred Strains, Pilot Projects, Tissue density, Dysferlin, Mice, Necrosis, Extracellular fluid, Organometallic Compounds, medicine, Animals, Radiology, Nuclear Medicine and imaging, Muscular dystrophy, Muscle, Skeletal, Fluorocarbons, biology, medicine.diagnostic_test, business.industry, Age Factors, Gadofluorine M, Membrane Proteins, Magnetic resonance imaging, Muscular Dystrophy, Animal, medicine.disease, Magnetic Resonance Imaging, Mr imaging, Mice, Inbred C57BL, Permeability surface, Microscopy, Fluorescence, biology.protein, business, Extravasation of Diagnostic and Therapeutic Materials |
| الوصف: | To compare the usefulness of gadofluorine M with that of Gadomer in assessment of dysferlin-deficient muscular dystrophy at 7.0-T magnetic resonance (MR) imaging.All experiments were approved by local review boards. SJL/J mice (n = 24) with dysferlin-deficient muscular dystrophy and C57BL/6 control mice (n = 24) were imaged at 12-15 weeks (young) or older than 30 weeks (old) by using dynamic contrast material-enhanced imaging with inversion-prepared steady-state free-precession sequence before, during, and after administration of gadofluorine M at 2 micromol or Gadomer at 4 micromol intravenously. After imaging, regions of interest were determined from the upper extremity and left ventricular chamber; fractional extravascular extracellular volume, v(e), and permeability surface tissue density product, PS rho, were measured by using a two-compartment pharmacokinetic model. The natural history of muscular dystrophy was assessed histologically in 70 mice (seven five-mouse groups each of SJL/J mice and of control mice) at 4-week intervals from 8 to 32 weeks. In addition, three SJL/J mice and three control mice at age 33 weeks were sacrificed, and fluorescence microscopy was performed for visualization of intravenously administered carbocyanine-labeled gadofluorine M in muscle cells. Statistical analysis was performed by using the t test.Gadofluorine M enhancement was significantly greater in skeletal muscle of 30-week-old mice with dysferlin-deficient muscular dystrophy, compared with control mice. Gadofluorine M demonstrated both increased rate of enhancement (PS rho sec(-1) +/- standard error of the mean: 0.004 e(-)(4) +/- 3 vs 0.002 e(-)(4) +/- 3; P.05) and increased level of enhancement (v(e) +/- standard error of the mean: 0.035 +/- 0.004 vs 0.019 +/- 0.004; P.05). Gadomer showed no differential enhancement in the two mouse groups. Histologic examination confirmed the presence of labeled gadofluorine M in muscle cells.Gadofluorine M-enhanced MR imaging may be of value in monitoring dysferlin-deficient muscular dystrophy disease progression in this animal model and could prove to be a useful tool in following the course of chronic muscle diseases in humans. |
| تدمد: | 1527-1315 0033-8419 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb62b2c49448c5772b53d32a1f7a90f4 https://doi.org/10.1148/radiol.2501080180 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bb62b2c49448c5772b53d32a1f7a90f4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15271315 00338419 |
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