AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
| العنوان: | AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex |
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| المؤلفون: | Royce A. Wilkinson, Wang-Ting Lu, Gabriel C. Lander, Sarah Golden, Andrew Santiago-Frangos, Marscha Hirschi, Alan R. Davidson, Blake Wiedenheft |
| المصدر: | Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-6 (2020) |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Models, Molecular, 0301 basic medicine, Protein Conformation, Science, General Physics and Astronomy, Computational biology, Plasma protein binding, Proteus penneri, Biochemistry, Article, General Biochemistry, Genetics and Molecular Biology, Viral Proteins, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Immune system, Protein structure, Bacterial Proteins, CRISPR, Bacteriophages, Amino Acid Sequence, lcsh:Science, Peptide sequence, Multidisciplinary, Bacteria, Sequence Homology, Amino Acid, biology, Chemistry, Cryoelectron Microscopy, RNA, DNA, General Chemistry, biology.organism_classification, 030104 developmental biology, Multiprotein Complexes, Nucleic Acid Conformation, lcsh:Q, CRISPR-Cas Systems, 030217 neurology & neurosurgery, Protein Binding, RNA, Guide, Kinetoplastida |
| الوصف: | Bacteria have evolved sophisticated adaptive immune systems, called CRISPR-Cas, that provide sequence-specific protection against phage infection. In turn, phages have evolved a broad spectrum of anti-CRISPRs that suppress these immune systems. Here we report structures of anti-CRISPR protein IF9 (AcrIF9) in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy). In addition to sterically blocking the hybridization of complementary dsDNA to the CRISPR RNA, our results show that AcrIF9 binding also promotes non-sequence-specific engagement with dsDNA, potentially sequestering the complex from target DNA. These findings highlight the versatility of anti-CRISPR mechanisms utilized by phages to suppress CRISPR-mediated immune systems. The anti-CRISPR protein IF9 (AcrIF9) specifically inhibits the type I-F CRISPR adaptive immune system. Here, the authors present the cryo-EM structure of AcrIF9 in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy) and a dsDNA bound Csy-AcrIF9 structure, and find that AcrIF9 binding to the Csy complex triggers non-sequence specific dsDNA binding to Csy-AcrIF9, which might sequester the complex from its target DNA. |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb9efb2cd6872ad952d47cd50dcd6b26 https://doi.org/10.1038/s41467-020-16512-1 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bb9efb2cd6872ad952d47cd50dcd6b26 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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