Acute cigarette smoke inhalation blunts lung responsiveness to methacholine and allergen in rabbit: differentiation of central and peripheral effects
| العنوان: | Acute cigarette smoke inhalation blunts lung responsiveness to methacholine and allergen in rabbit: differentiation of central and peripheral effects |
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| المؤلفون: | Satu Strengell, Walid Habre, Kimmo Neitola, Ferenc Peták, Pekka Suortti, Sam Bayat, Heikki Suhonen, Anssi Sovijärvi, Liisa Porra, Tibor Z. Jánosi |
| المصدر: | American Journal of Physiology. Lung Cellular and Molecular Physiology, Vol. 299, No 2 (2010) pp. L242-251 |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Male, Physiology, Ovalbumin, Allergens/pharmacology, Methacholine Chloride/pharmacology, 030204 cardiovascular system & hematology, medicine.disease_cause, Tobacco smoke, 03 medical and health sciences, 0302 clinical medicine, Allergen, Physiology (medical), Administration, Inhalation, medicine, Lung/drug effects/*immunology/physiology/radiography, Animals, Chymotrypsin, Respiratory system, Lung, Methacholine Chloride, 030304 developmental biology, Asthma, 0303 health sciences, Carbon Monoxide, ddc:617, Inhalation, business.industry, Respiratory disease, Smoking, Cell Biology, respiratory system, Allergens, medicine.disease, Carbon Monoxide/adverse effects, Ovalbumin/immunology, respiratory tract diseases, medicine.anatomical_structure, Anesthesia, Immunology, Chymotrypsin/pharmacology, Smoking/*adverse effects, Methacholine, Rabbits, business, Tomography, X-Ray Computed, medicine.drug |
| الوصف: | Despite the prevalence of active smoking in asthmatics, data on the short-term effect of acute mainstream tobacco smoke exposure on airway responsiveness are very scarce. The aim of this study was to assess the immediate effect of acute exposure to mainstream cigarette smoke on airway reactivity to subsequent nonspecific and allergenic challenges in healthy control ( n = 5) and ovalbumin-sensitized rabbits ( n = 6). We combined low-frequency forced oscillations and synchrotron radiation CT imaging to differentiate central airway and peripheral airway and lung parenchymal components of the response to airway provocation. Acute exposure to smoke generated by four successive cigarettes (CS) strongly inhibited the central airway response to subsequent IV methacholine (MCh) challenge. In the sensitized animals, although the response to ovalbumin was also inhibited in the central airways, mainstream CS did not blunt the peripheral airway response in this group. In additional groups of experiments, exposure to HEPA-filtered CS ( n = 6) similarly inhibited the MCh response, whereas CO (10,000 ppm for 4 min, n = 6) or nitric oxide inhalation instead of CS (240 ppm, 4 × 7 min, n = 5) failed to blunt nonspecific airway responsiveness. Pretreatment with α-chymotrypsin to inhibit endogenous VIP before CS exposure had no effect ( n = 4). Based on these observations, the gas phase of mainstream cigarette smoke may contain one or more short-term inhibitory components acting primarily on central airways and inhibiting the response to both specific and nonspecific airway provocation, but not on the lung periphery where both lung mechanical parameters, and synchrotron-imaging derived parameters, showed large changes in response to allergen challenge in sensitized animals. |
| تدمد: | 1522-1504 1040-0605 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbc04cff65448e4d12cf5470210df52e https://pubmed.ncbi.nlm.nih.gov/20543004 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bbc04cff65448e4d12cf5470210df52e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221504 10400605 |
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