IFN-γ Drives human monocyte differentiation into highly proinflammatory macrophages that resemble a phenotype relevant to psoriasis
| العنوان: | IFN-γ Drives human monocyte differentiation into highly proinflammatory macrophages that resemble a phenotype relevant to psoriasis |
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| المؤلفون: | William L. Thompson, Rab K. Prinjha, Rosario Luque-Martin, Inmaculada Rioja, Menno P.J. de Winther, Sharon G. Bernard, Mathias Kalxdorf, Charlotte A. Ashby, Jan Van den Bossche, Catriona Sharp, Palwinder K. Mander, Annette E. Neele, Wouter J. de Jonge, Davina C. Angell, H. Christian Eberl, Johannes Freudenberg |
| المساهمون: | Molecular cell biology and Immunology, ACS - Atherosclerosis & ischemic syndromes, Amsterdam Gastroenterology Endocrinology Metabolism, Graduate School, ACS - Pulmonary hypertension & thrombosis, AII - Inflammatory diseases, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, ARD - Amsterdam Reproduction and Development, Medical Biochemistry, Gastroenterology and Hepatology, Tytgat Institute for Liver and Intestinal Research |
| المصدر: | Luque-Martin, R, Angell, D C, Kalxdorf, M, Bernard, S, Thompson, W, Eberl, H C, Ashby, C, Freudenberg, J, Sharp, C, van den Bossche, J, de Jonge, W J, Rioja, I, Prinjha, R K, Neele, A E, de Winther, M P J & Mander, P K 2021, ' IFN-γ Drives human monocyte differentiation into highly proinflammatory macrophages that resemble a phenotype relevant to psoriasis ', Journal of Immunology, vol. 207, no. 2, pp. 555-568 . https://doi.org/10.4049/jimmunol.2001310 Journal of Immunology, 207(2), 555-568. American Association of Immunologists Journal of immunology (Baltimore, Md., 207(2), 555-568. American Association of Immunologists |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Proteomics, Immunology, Biology, Monocytes, Proinflammatory cytokine, Interferon-gamma, Immune system, Psoriasis, medicine, Humans, Immunology and Allergy, Macrophage, Cells, Cultured, Skin, Inflammation, Macrophage Colony-Stimulating Factor, Macrophages, Monocyte, Cell Differentiation, medicine.disease, Phenotype, In vitro, Cell biology, medicine.anatomical_structure, Monocyte differentiation, Biomarkers |
| الوصف: | As key cells of the immune system, macrophages coordinate the activation and regulation of the immune response. Macrophages present a complex phenotype that can vary from homeostatic, proinflammatory, and profibrotic to anti-inflammatory phenotypes. The factors that drive the differentiation from monocyte to macrophage largely define the resultant phenotype, as has been shown by the differences found in M-CSF– and GM-CSF–derived macrophages. We explored alternative inflammatory mediators that could be used for in vitro differentiation of human monocytes into macrophages. IFN-γ is a potent inflammatory mediator produced by lymphocytes in disease and infections. We used IFN-γ to differentiate human monocytes into macrophages and characterized the cells at a functional and proteomic level. IFN-γ alone was sufficient to generate macrophages (IFN-γ Mϕ) that were phagocytic and responsive to polarization. We demonstrate that IFN-γ Mϕ are potent activators of T lymphocytes that produce IL-17 and IFN-γ. We identified potential markers (GBP-1, IP-10, IL-12p70, and IL-23) of IFN-γ Mϕ and demonstrate that these markers are enriched in the skin of patients with inflamed psoriasis. Collectively, we show that IFN-γ can drive human monocyte to macrophage differentiation, leading to bona fide macrophages with inflammatory characteristics. |
| اللغة: | English |
| تدمد: | 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbc517f304ed2fe12bc5eeb2aa48c676 https://research.vumc.nl/en/publications/469e0fd3-afc0-42e4-9c7d-f61ecc14c6aa |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....bbc517f304ed2fe12bc5eeb2aa48c676 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00221767 |
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