The Paraguayan Rhinella toad venom: Implications in the traditional medicine and proliferation of breast cancer cells
| العنوان: | The Paraguayan Rhinella toad venom: Implications in the traditional medicine and proliferation of breast cancer cells |
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| المؤلفون: | Miriam Rolón, Flavia Netto, Francisco Brusquetti, Alberto Burgos-Edwards, Celeste Vega Gómez, Antonieta Rojas de Arias, Guillermo Schmeda-Hirschmann, Félix A. Urra, César Cárdenas, Jorge Alfonso |
| المصدر: | J Ethnopharmacol Artículos CONICYT CONICYT Chile instacron:CONICYT |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.drug_class, Venom, Breast Neoplasms, Toad, 03 medical and health sciences, Mice, 0302 clinical medicine, Rhinella schneideri, biology.animal, Cell Line, Tumor, Drug Discovery, medicine, Animals, Humans, Cells, Cultured, Cell Proliferation, Pharmacology, integumentary system, biology, Traditional medicine, Rhinella ornata, Macrophages, Cancer, biology.organism_classification, medicine.disease, 030104 developmental biology, Paraguay, 030220 oncology & carcinogenesis, Antiprotozoal, Rhinella, Amphibian Venoms, Bufo marinus, Female, Medicine, Traditional, Rhinella scitula |
| الوصف: | Ethnopharmacological relevance Toads belonging to genus Rhinella are used in Paraguayan traditional medicine to treat cancer and skin infections. Aim of the study The objective of the study was to determine the composition of venoms obtained from three different Paraguayan Rhinella species, to establish the constituents of a preparation sold in the capital city of Paraguay to treat cancer as containing the toad as ingredient, to establish the effect of the most active Rhinella schneideri venom on the cell cycle using human breast cancer cells and to assess the antiprotozoal activity of the venoms. Methods The venom obtained from the toads parotid glands was analyzed by HPLC-MS-MS. The preparation sold in the capital city of Paraguay to treat cancer that is advertised as made using the toad was analyzed by HPLC-MS-MS. The effect of the R. schneideri venom and the preparation was investigated on human breast cancer cells. The antiprotozoal activity was evaluated on Leishmania braziliensis , L . infantum and murine macrophages. Results From the venoms of R. ornata , R. schneideri and R. scitula , some 40 compounds were identified by spectroscopic and spectrometric means. Several minor constituents are reported for the first time. The preparation sold as made from the toad did not contained bufadienolides or compounds that can be associated with the toad but plant compounds, mainly phenolics and flavonoids. The venom showed activity on human breast cancer cells and modified the cell cycle proliferation. The antiprotozoal effect was higher for the R. schneideri venom and can be related to the composition and relative ratio of constituents compared with R. ornata and R. scitula . Conclusions The preparation sold in the capital city of Paraguay as containing the toad venom, used popularly to treat cancer did not contain the toad venom constituents. Consistent with this, this preparation was inactive on proliferation of human breast cancer cells. In contrast, the toad venoms of Rhinella species altered the cell cycle progression, affecting the proliferation of malignant cells. The findings suggest that care should be taken with the providers of the preparation and that the crude drug present a strong activity towards human breast cancer cell lines. The antiprotozoal effect of the R. schneideri venom was moderate while the venom of R. ornata was devoid of activity and that of R. scitula was active at very high concentration. |
| تدمد: | 1872-7573 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbcf153c7c96d26c460f058e88c0aa79 https://pubmed.ncbi.nlm.nih.gov/28131913 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bbcf153c7c96d26c460f058e88c0aa79 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18727573 |
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