Palmitate enhanced the cytotoxicity of ZnO nanomaterials possibly by promoting endoplasmic reticulum stress
| العنوان: | Palmitate enhanced the cytotoxicity of ZnO nanomaterials possibly by promoting endoplasmic reticulum stress |
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| المؤلفون: | Jimin Long, Juan Li, Yi Cao, Ting Yang, Yanhuai Ding, Jiamao Chen, Xianqiang Li |
| المصدر: | Journal of Applied Toxicology. 39:798-806 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Thapsigargin, Cell Survival, Surface Properties, THP-1 Cells, Palmitates, 010501 environmental sciences, Toxicology, 01 natural sciences, Monocytes, 03 medical and health sciences, chemistry.chemical_compound, Humans, Inducer, Cytotoxicity, Endoplasmic Reticulum Chaperone BiP, 030304 developmental biology, 0105 earth and related environmental sciences, chemistry.chemical_classification, 0303 health sciences, Reactive oxygen species, Dose-Response Relationship, Drug, Chemistry, Endoplasmic reticulum, Drug Synergism, Endoplasmic Reticulum Stress, humanities, Apoptosis, Unfolded protein response, Biophysics, Nanoparticles, Zinc Oxide, Reactive Oxygen Species, human activities, Intracellular |
| الوصف: | We recently synthesized ZnO nanomaterials (denoted as ZnO nanorods [NRs] and Mini-NRs) and suggested that their cytotoxicity could be related with the activation of endoplasmic reticulum (ER) stress apoptosis. However, in a complex biological microenvironment, the ER stress-apoptosis pathway could also be modulated by biological molecules, such as free fatty acids, leading to unpredicted biological effects. In this study, we investigated the combined toxicity of ZnO NRs/Mini-NRs and palmitate (PA) to THP-1 macrophages. PA influenced the zeta potential and solubility of ZnO NRs and ZnO Mini-NRs in water, which indicated a change of colloidal stability. Exposure to ZnO NRs and Mini-NRs dose-dependent decreased cellular viability and release of soluble monocyte chemotactic protein 1 (sMCP-1), and these effects were significantly promoted with the presence of PA. However, ZnO NR- and Mini-NR-induced intracellular Zn ions or reactive oxygen species were not significantly affected by PA. ZnO NRs and ZnO Mini-NRs significantly promoted the expression of ER stress genes HSPA5, DDIT3, XBP-1s and apoptotic gene CASP3, whereas PA also modestly promoted the expression of HSPA5, DDIT3 and CASP3. Interestingly, the ER stress inducer thapsigargin showed a similar effect as PA to promote the cytotoxicity of ZnO NRs and ZnO Mini-NRs. It is suggested that PA might promote the cytotoxicity of ZnO NRs and ZnO Mini-NRs possibly by promoting ER stress. |
| تدمد: | 1099-1263 0260-437X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc15ee33a15cd7bf755c61e5524e9a84 https://doi.org/10.1002/jat.3768 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....bc15ee33a15cd7bf755c61e5524e9a84 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10991263 0260437X |
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