A metabolic pathway for catabolizing levulinic acid in bacteria
| العنوان: | A metabolic pathway for catabolizing levulinic acid in bacteria |
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| المؤلفون: | Daniel E. Agnew, Kelly M. Wetmore, Tippapha Pisithkul, Ryan L. Clark, Daniel Amador-Noguez, Morgan N. Price, Jacqueline M. Rand, Joshua M. Thiede, Christopher R. Mehrer, Yumi Suh, Andrew L. Markley, Candace E. Campbell, Adam P. Arkin, Brian F. Pfleger, Adam M. Deutschbauer, Jayashree Ray |
| المصدر: | Nature microbiology Rand, JM; Pisithkul, T; Clark, RL; Thiede, JM; Mehrer, CR; Agnew, DE; et al.(2017). A metabolic pathway for catabolizing levulinic acid in bacteria. Nature Microbiology, 2(12), 1624-1634. doi: 10.1038/s41564-017-0028-z. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/7wj2z10w |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Immunology, Microbial metabolism, Lignocellulosic biomass, 7. Clean energy, Applied Microbiology and Biotechnology, Microbiology, Article, Metabolic engineering, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, Operon, Escherichia coli, Genetics, Levulinic acid, Biomass, Biorefining, Base Sequence, Bacteria, biology, Pseudomonas putida, Catabolism, Gene Expression Regulation, Bacterial, Cell Biology, biology.organism_classification, Carbon, Levulinic Acids, Metabolic pathway, 030104 developmental biology, Metabolic Engineering, Biochemistry, chemistry, Genes, Bacterial, Gene Knockdown Techniques, DNA Transposable Elements, CRISPR-Cas Systems, Propionates, Metabolic Networks and Pathways |
| الوصف: | © 2017 The Author(s). Microorganisms can catabolize a wide range of organic compounds and therefore have the potential to perform many industrially relevant bioconversions. One barrier to realizing the potential of biorefining strategies lies in our incomplete knowledge of metabolic pathways, including those that can be used to assimilate naturally abundant or easily generated feedstocks. For instance, levulinic acid (LA) is a carbon source that is readily obtainable as a dehydration product of lignocellulosic biomass and can serve as the sole carbon source for some bacteria. Yet, the genetics and structure of LA catabolism have remained unknown. Here, we report the identification and characterization of a seven-gene operon that enables LA catabolism in Pseudomonas putida KT2440. When the pathway was reconstituted with purified proteins, we observed the formation of four acyl-CoA intermediates, including a unique 4-phosphovaleryl-CoA and the previously observed 3-hydroxyvaleryl-CoA product. Using adaptive evolution, we obtained a mutant of Escherichia coli LS5218 with functional deletions of fadE and atoC that was capable of robust growth on LA when it expressed the five enzymes from the P. putida operon. This discovery will enable more efficient use of biomass hydrolysates and metabolic engineering to develop bioconversions using LA as a feedstock. |
| وصف الملف: | application/pdf |
| تدمد: | 2058-5276 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bca4ff3609553c375087abc854e6f6ad https://doi.org/10.1038/s41564-017-0028-z |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bca4ff3609553c375087abc854e6f6ad |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20585276 |
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