Phage-Based Profiling of Rare Single Cells Using Nanoparticle-Directed Capture
| العنوان: | Phage-Based Profiling of Rare Single Cells Using Nanoparticle-Directed Capture |
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| المؤلفون: | Shana O. Kelley, Hansen Wang, Fan Xia, Bill Duong, Lia Cardarelli, Sharif Uddin Ahmed, Kangfu Chen, Sachdev S. Sidhu, Zongjie Wang, Irene Lui, James A. Wells, Randy Atwal, Yuan Ma |
| المصدر: | ACS Nano. 15:19202-19210 |
| بيانات النشر: | American Chemical Society (ACS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | In situ, Microfluidics, General Physics and Astronomy, Nanoparticle, Cell Separation, 02 engineering and technology, Computational biology, 03 medical and health sciences, Circulating tumor cell, Antigen, Cell Line, Tumor, Tumor Microenvironment, Humans, Bacteriophages, General Materials Science, 030304 developmental biology, Profiling (computer programming), 0303 health sciences, Chemistry, General Engineering, Neoplastic Cells, Circulating, 021001 nanoscience & nanotechnology, Proteome, Protein Expression Analysis, Nanoparticles, 0210 nano-technology |
| الوصف: | Advances in single-cell level profiling of the proteome require quantitative and versatile platforms, especially for rare cell analyses such as circulating tumor cell (CTC) profiling. Here we demonstrate an integrated microfluidic chip that uses magnetic nanoparticles to capture single tumor cells with high efficiency, permits on-chip incubation, and facilitates in situ cell-surface protein expression analysis. Combined with phage-based barcoding and next-generation sequencing technology, we were able to monitor changes in the expression of multiple surface markers stimulated in response to CTC adherence. Interestingly, we found fluctuations in the expression of Frizzled2 (FZD2) that reflected the microenvironment of the single cells. This platform has a high potential for in-depth screening of multiple surface antigens simultaneously in rare cells with single-cell resolution, which will provide further insights regarding biological heterogeneity and human disease. |
| تدمد: | 1936-086X 1936-0851 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bcb24fcbd28a8223b115078b27f78485 https://doi.org/10.1021/acsnano.1c03935 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....bcb24fcbd28a8223b115078b27f78485 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 1936086X 19360851 |
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