Fibroblast growth factor 23 production in bone is directly regulated by 1α,25-dihydroxyvitamin D, but not PTH
| العنوان: | Fibroblast growth factor 23 production in bone is directly regulated by 1α,25-dihydroxyvitamin D, but not PTH |
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| المؤلفون: | Yuka Maeda, Toru Mima, Takashi Shigematsu, Shigeo Negi, Masaki Ohya, Fumie Saji, Hikari Orita, Toshifumi Sakaguchi, Maki Ooura |
| المصدر: | American Journal of Physiology-Renal Physiology. 299:F1212-F1217 |
| بيانات النشر: | American Physiological Society, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Male, Fibroblast growth factor 23, medicine.medical_specialty, Physiology, urologic and male genital diseases, Nephrectomy, Calcitriol receptor, Bone and Bones, Rats, Sprague-Dawley, chemistry.chemical_compound, Calcitriol, Internal medicine, medicine, Animals, RNA, Messenger, Uremia, 1α 25 dihydroxyvitamin d, Reverse Transcriptase Polymerase Chain Reaction, Body Weight, Skull, Metabolism, Phosphate, medicine.disease, Rats, Fibroblast Growth Factors, Calcium Channel Agonists, stomatognathic diseases, Endocrinology, chemistry, Parathyroid Hormone, Kidney Failure, Chronic, Receptors, Calcitriol, Secondary hyperparathyroidism, Renal phosphate excretion |
| الوصف: | Fibroblast growth factor 23 (FGF23), which is primarily produced by osteocytes in bone, regulates renal phosphate excretion and 1α,25-dihydroxyvitamin D [1,25(OH)2D3] metabolism. Patients with chronic kidney disease (CKD) have increased levels of circulating serum FGF23, but the direct effect on circulating FGF23 levels in renal insufficiency is still unclear. To identify the major regulator of FGF23 synthesis in renal insufficiency, we compared the effect of parathyroid hormone (PTH) and 1,25(OH)2D3 on FGF23 synthesis in the calvariae of normal rats with that of uremic rats in vitro. 1,25(OH)2D3 treatment significantly increased the FGF23 concentration in the medium from both groups, but the degree of increase in the uremic group was markedly higher than in the control group. A significant increase in FGF23 mRNA expression occurred as early as 4 h after treatment and reached the maximum within 8 h in the uremic group, whereas in the normal group a significant increase in FGF23 mRNA expression was observed only at 8 h. In addition, the expression of vitamin D receptor (VDR) mRNA in the calvariae of uremic rats was markedly higher than in normal rats. However, in neither group did PTH treatment affect the medium FGF23 concentration or the FGF23 mRNA levels. These results suggest that FGF23 synthesis in bone is regulated by 1,25(OH)2D3 directly, not by PTH, and that increased VDR mRNA expression induced the relatively swift and strong response in the uremic group. |
| تدمد: | 1522-1466 1931-857X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bcb922abcb2949dfb95b1038a3c362a0 https://doi.org/10.1152/ajprenal.00169.2010 |
| رقم الأكسشن: | edsair.doi.dedup.....bcb922abcb2949dfb95b1038a3c362a0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221466 1931857X |
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