Simple and rapid validated HPLC-fluorescence determination of perampanel in the plasma of patients with epilepsy
| العنوان: | Simple and rapid validated HPLC-fluorescence determination of perampanel in the plasma of patients with epilepsy |
|---|---|
| المؤلفون: | Carmina Candela, Manuela Contin, Susan Mohamed, Roberto Riva |
| المصدر: | Practical Laboratory Medicine, Vol 10, Iss C, Pp 15-20 (2018) Practical Laboratory Medicine |
| بيانات النشر: | Elsevier BV, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | AMPA, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, VPA, valproic acid, CBZ, carbamazepine, Antiepileptic drugs, Clinical Biochemistry, PHT, phenytoin, Clinical pharmacokinetics, LLOQ, lower limit of quantification, Pharmacology, Perampanel, 030226 pharmacology & pharmacy, Article, LLOD, lower limit of detection, lcsh:Chemistry, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Hplc fluorescence, TDM, therapeutic drug monitoring, medicine, Sample preparation, IS, internal standard, MS/MS, tandem mass spectrometer, Acetonitrile, lcsh:R5-920, Epilepsy, Chromatography, Radiological and Ultrasound Technology, medicine.diagnostic_test, AED, antiepileptic drug, Plasma, Fluorescence, LLE, liquid-liquid extraction, QC, quality control, lcsh:QD1-999, chemistry, Therapeutic drug monitoring, OXC, oxcarbazepine, PER, perampanel, HPLC-F, high performance liquid chromatography-fluorescence detector, MIR, mirtazapine, lcsh:Medicine (General), Sodium acetate, HPLC-F, 030217 neurology & neurosurgery |
| الوصف: | We present a simple and fast high-performance liquid chromatography method with fluorescence detection for the determination of the antiepileptic drug perampanel in human plasma. The chromatographic separation was performed on a Kinetex PFP (100 × 2.6 mm, 4.6 µm) column, using a mobile phase of sodium acetate 0.03 M pH 3.7 and acetonitrile (40/60, v/v), at a flow rate of 0.8 mL/min. Total chromatography time for each run was 5 min. Sample preparation (250 µL) involved only one simple precipitation step by acetonitrile spiked with mirtazapine as internal standard. The method was validated over a concentration range of 20–1000 ng/mL and successfully applied to measure perampanel concentrations in plasma samples obtained from patients with epilepsy. This assay combines the high specificity of fluorescence detection with a very simple and fast sample pretreatment and can offer real advantages over existing methods in terms of simplicity and transferability to a therapeutic drug monitoring setting. Highlights • A very simple assay for the determination of perampanel in human plasma is proposed. • The analysis is based on HPLC-F coupled with an only deproteinization step. • No time consuming liquid-liquid extractions and evaporations are required. • This assay significantly simplifies existing ones in terms of transferability to TDM. |
| تدمد: | 2352-5517 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd7ce060897a1c004dfcadfca9820bf3 https://doi.org/10.1016/j.plabm.2017.11.003 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bd7ce060897a1c004dfcadfca9820bf3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23525517 |
|---|