Cortical contraction drives the 3D patterning of epithelial cell surfaces
| العنوان: | Cortical contraction drives the 3D patterning of epithelial cell surfaces |
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| المؤلفون: | Goryachev, Maryshev, Aaron P van Loon, Sagasti, Erofeev |
| المصدر: | The Journal of cell biology, vol 219, iss 3 van Loon, A P, Erofeev, I, Maryshev, I, Goryachev, A & Sagasti, A 2020, ' Cortical contraction drives the 3D patterning of epithelial cell surfaces ', Journal of Cell Biology, vol. 219, no. 3, e201904144 . https://doi.org/10.1083/jcb.201904144 The Journal of Cell Biology |
| بيانات النشر: | Rockefeller University Press, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Time Factors, Contraction (grammar), Morphogenesis, Genetically Modified, Development, Medical and Health Sciences, Article, Animals, Genetically Modified, 03 medical and health sciences, 0302 clinical medicine, Live cell imaging, Myosin, medicine, Animals, Surface Tension, Zebrafish, Cytoskeleton, Actin, Skin, 030304 developmental biology, Myosin Type II, 0303 health sciences, biology, Chemistry, Epithelial Cells, Apical constriction, Actomyosin, Cell Biology, Zebrafish Proteins, Biological Sciences, Complex cell, biology.organism_classification, Epithelium, Biomechanical Phenomena, Actin Cytoskeleton, medicine.anatomical_structure, Biophysics, Cell Surface Extensions, 030217 neurology & neurosurgery, Developmental Biology |
| الوصف: | Microridges, elongated 3D protrusions arranged in maze-like patterns on zebrafish skin cells, form by the accretion of simple precursor projections. Modeling and in vivo experiments showed that cortical contractions promote the coalescence of precursors into microridges by reducing surface tension. Cellular protrusions create complex cell surface topographies, but biomechanical mechanisms regulating their formation and arrangement are largely unknown. To study how protrusions form, we focused on the morphogenesis of microridges, elongated actin-based structures that are arranged in maze-like patterns on the apical surfaces of zebrafish skin cells. Microridges form by accreting simple finger-like precursors. Live imaging demonstrated that microridge morphogenesis is linked to apical constriction. A nonmuscle myosin II (NMII) reporter revealed pulsatile contractions of the actomyosin cortex, and inhibiting NMII blocked apical constriction and microridge formation. A biomechanical model suggested that contraction reduces surface tension to permit the fusion of precursors into microridges. Indeed, reducing surface tension with hyperosmolar media promoted microridge formation. In anisotropically stretched cells, microridges formed by precursor fusion along the stretch axis, which computational modeling explained as a consequence of stretch-induced cortical flow. Collectively, our results demonstrate how contraction within the 2D plane of the cortex can pattern 3D cell surfaces. |
| وصف الملف: | application/pdf |
| تدمد: | 1540-8140 0021-9525 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd882b598ed8406dd0922402b7e843c6 https://doi.org/10.1083/jcb.201904144 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bd882b598ed8406dd0922402b7e843c6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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