A novel bivalent Pasteurellosis-RHD vaccine candidate adjuvanted with Montanide ISA70 protects rabbits from lethal challenge
| العنوان: | A novel bivalent Pasteurellosis-RHD vaccine candidate adjuvanted with Montanide ISA70 protects rabbits from lethal challenge |
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| المؤلفون: | Mahmoud A. Elgamal, Saleh A. Kabli, Basem M. Ahmed, Jakeen K. El-Jakee, Khalid S. Almaary, Mai S. Omran, Hassan A. Hemeg, Jwaher Haji Alhaaji, Ayman S. Mubarak, Ihab M. Moussa, Sherif Marouf |
| المصدر: | Saudi Journal of Biological Sciences Saudi Journal of Biological Sciences, Vol 27, Iss 3, Pp 996-1001 (2020) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Montanide ISA70, Vaccination schedule, Bivalent, 01 natural sciences, Bivalent (genetics), Article, 03 medical and health sciences, Medicine, Pasteurella multocida, lcsh:QH301-705.5, Hemagglutination assay, biology, business.industry, RHDV, medicine.disease, biology.organism_classification, Virology, Pasteurellosis, Vaccination, Titer, 030104 developmental biology, lcsh:Biology (General), biology.protein, Rabbits, Antibody, General Agricultural and Biological Sciences, business, Vaccine, 010606 plant biology & botany |
| الوصف: | In the present study, a bivalent vaccine against Pasteurella multocida and rabbit hemorrhagic disease virus (RHDV) was formulated with Montanide™ ISA70 oil adjuvant (Seppic, Paris, France). Its efficacy was evaluated and compared to similar monovalent preparations and commercially available monovalent vaccines. White new Zeeland rabbit groups (n = 10) received 2 successive doses of the tested vaccines and were challenged 2 weeks after 2nd dose with Pasteurella multocida and RHDV or either pathogens according to their vaccination schedule. Challenged not-vaccinated group of rabbits (n = 10) was included as a control. The bivalent and monovalent ISA70 preparations were found stable, safe, sterile, pure and of low viscosity. Group 3 (GP3) which received bivalent vaccine showed the highest antibody geometric mean titers against Pasteurella multocida and RHDV evaluated by ELISA and hemagglutination inhibition (HI) respectively. Following virulent challenge; Gp3 rabbits were 90% protected from challenge over other groups that showed 80% protection. Detection of either pathogen in the livers of dead and euthanized rabbits had failed except for non-vaccinated controls. The bivalent vaccine candidate was fully protective. Immunization against both pathogens can be achieved by single vaccination. Keywords: Pasteurellosis, RHDV, Vaccine, Rabbits, Bivalent, Montanide ISA70 |
| تدمد: | 1319-562X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be530c23051ac3867e57c8132e5188dc https://pubmed.ncbi.nlm.nih.gov/32127779 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....be530c23051ac3867e57c8132e5188dc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1319562X |
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