Sequence of cDNA for rat cystathionine γ-lyase and comparison of deduced amino acid sequence with related Escherichia coli enzymes
| العنوان: | Sequence of cDNA for rat cystathionine γ-lyase and comparison of deduced amino acid sequence with related Escherichia coli enzymes |
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| المؤلفون: | L. M. Glode, P. F. Erickson, Marc Baumann, Lih-Jen Su, I. H. Maxwell |
| المصدر: | Biochemical Journal. 269:335-340 |
| بيانات النشر: | Portland Press Ltd., 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Carbon-Oxygen Lyases, Molecular Sequence Data, Lyases, medicine.disease_cause, Biochemistry, 03 medical and health sciences, 0302 clinical medicine, Sequence Homology, Nucleic Acid, Complementary DNA, Escherichia coli, medicine, Animals, Genomic library, Amino Acid Sequence, Cloning, Molecular, Molecular Biology, Peptide sequence, Immunosorbent Techniques, 030304 developmental biology, 0303 health sciences, Base Sequence, biology, cDNA library, Cystathionine gamma-lyase, Cystathionine gamma-Lyase, Nucleic acid sequence, Nucleic Acid Hybridization, DNA, Cell Biology, Cystathionine beta synthase, Molecular biology, Rats, biology.protein, Electrophoresis, Polyacrylamide Gel, 030217 neurology & neurosurgery, Research Article |
| الوصف: | A cDNA clone for cystathionine gamma-lyase was isolated from a rat cDNA library in lambda gt11 by screening with a monospecific antiserum. The identity of this clone, containing 600 bp proximal to the 3′-end of the gene, was confirmed by positive hybridization selection. Northern-blot hybridization showed the expected higher abundance of the corresponding mRNA in liver than in brain. Two further cDNA clones from a plasmid pcD library were isolated by colony hybridization with the first clone and were found to contain inserts of 1600 and 1850 bp. One of these was confirmed as encoding cystathionine gamma-lyase by hybridization with two independent pools of oligodeoxynucleotides corresponding to partial amino acid sequence information for cystathionine gamma-lyase. The other clone (estimated to represent all but 8% of the 5′-end of the mRNA) was sequenced and its deduced amino acid sequence showed similarity to those of the Escherichia coli enzymes cystathionine beta-lyase and cystathionine gamma-synthase throughout its length, especially to that of the latter. |
| تدمد: | 1470-8728 0264-6021 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be9c2be1feaaad66feb2233017f0c52a https://doi.org/10.1042/bj2690335 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....be9c2be1feaaad66feb2233017f0c52a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14708728 02646021 |
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