Changes of lysosomal membrane permeabilization and lipid metabolism in sidt2 deficient mice
| العنوان: | Changes of lysosomal membrane permeabilization and lipid metabolism in sidt2 deficient mice |
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| المؤلفون: | Lizhuo Wang, Yu Meng, Liefeng Ling |
| المصدر: | Experimental and Therapeutic Medicine |
| بيانات النشر: | D.A. Spandidos, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, biology, Liver cell, Aspartate transaminase, Lipid metabolism, SID1 transmembrane family member 2−/− mice, General Medicine, Articles, medicine.disease, Apoptotic body, Molecular biology, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, Immunology and Microbiology (miscellaneous), chemistry, Alanine transaminase, Lipid droplet, lipid metabolism, medicine, biology.protein, Oil Red O, lysosomal membrane permeabilization, Steatosis |
| الوصف: | The SID1 transmembrane family member 2 (sidt2) deficient mouse model was used to investigate the function of sidt2 in lysosomal membrane permeabilization and lipid metabolism of liver tissue. The mouse model was established by Cre/LoxP technology. Enzymatic methods were used to analyze the sidt2-/- mouse serum lipids, aspartate transaminase, alanine transaminase and serum bilirubin, compared with sidt2+/+ mice. Defective lipid metabolism and damaged liver functions were observed in the sidt2-/- mice. By using hematoxylin and eosin and Oil Red O staining, changes of morphology were observed in sidt2-/- mice with optical microscopy. Transmission electron microscopy was also used. Hepatic steatosis and partial liver tissue apoptosis were observed. The tissue distribution of sidt2 protein and mRNA was measured in knockout mice. The results indicated that negligible sidt2 mRNA and protein expression were observed in sidt2-/- mice, and that sidt2-/- mice had abnormal liver functions. Transmission electron microscopy revealed membrane lipid droplets in the liver cell cytoplasm, and some apoptotic body formation. These results demonstrated that absence of the lysosomal membrane protein sidt2 led to changes in lysosomal membrane permeabilization and lipid metabolism. |
| اللغة: | English |
| تدمد: | 1792-1015 1792-0981 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf514d318e0ff0f5d9160c0ee5b537e5 http://europepmc.org/articles/PMC5995057 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bf514d318e0ff0f5d9160c0ee5b537e5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921015 17920981 |
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