Non-adrenergic vasoconstriction and vasodilatation of guinea-pig aorta by β-phenylethylamine and amphetamine - Role of nitric oxide determined with L-NAME and NO scavengers
| العنوان: | Non-adrenergic vasoconstriction and vasodilatation of guinea-pig aorta by β-phenylethylamine and amphetamine - Role of nitric oxide determined with L-NAME and NO scavengers |
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| المؤلفون: | Harrison D. Broadley, Kenneth J. Broadley |
| المصدر: | European journal of pharmacology. 818 |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Agonist, Male, Curcumin, medicine.drug_class, Guinea Pigs, Vasodilation, Pharmacology, Xanthophylls, Nitric Oxide, Nitric oxide, 03 medical and health sciences, chemistry.chemical_compound, Phenylephrine, 0302 clinical medicine, Phenethylamines, Prazosin, medicine, Animals, Trace amine-associated receptor, Aorta, biology, food and beverages, Nitric oxide synthase, Amphetamine, 030104 developmental biology, NG-Nitroarginine Methyl Ester, chemistry, Vasoconstriction, biology.protein, medicine.symptom, 030217 neurology & neurosurgery, medicine.drug |
| الوصف: | Sympathomimetic and trace amines, including β-phenylethylamine (PEA) and amphetamine,\ud increase blood pressure and constrict isolated blood vessels. By convention this is regarded as\ud a sympathomimetic response, however, recent studies suggest trace amine-associated\ud receptor (TAAR) involvement. There is also uncertainty whether these amines also release\ud nitric oxide (NO) causing opposing vasodilatation. These questions were addressed in guineapig\ud isolated aorta, a species not previously examined. Guinea-pig aortic rings were set up to\ud measure contractile tension. Cumulative concentration-response curves were constructed for\ud the reference α-adrenoceptor agonist, phenylephrine, PEA or d-amphetamine before and in\ud the presence of vehicles, the α1-adrenoceptor antagonist, prazosin (1 μM), the nitric oxide\ud synthase inhibitor, Nω-nitro-L-arginine (L-NAME), or NO scavengers, curcumin and\ud astaxanthin. Prazosin inhibited phenylephrine contractions with low affinity consistent with\ud α1L-adrenoceptors. However, PEA and amphetamine were not antagonised, indicating nonadrenergic\ud responses probably via TAARs. L-NAME potentiated contractions to PEA both in\ud the absence and presence of prazosin, indicating that PEA releases NO to cause underlying\ud opposing vasodilatation, independent of α1-adrenoceptors. L-NAME also potentiated\ud amphetamine and phenylephrine. PEA was potentiated by the NO scavenger astaxanthin but\ud less effectively. Curcumin, an active component of turmeric, however, inhibited PEA. Trace\ud amines therefore constrict blood vessels non-adrenergically with an underlying NO-mediated\ud non-adrenergic vasodilatation. This has implications in the pressor actions of these amines\ud when NO is compromised. |
| وصف الملف: | application/pdf |
| تدمد: | 1879-0712 0014-2999 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfa8b5133942dd722f78773ff7a8e002 https://pubmed.ncbi.nlm.nih.gov/29074414 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bfa8b5133942dd722f78773ff7a8e002 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18790712 00142999 |
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