Targeted lung cancer therapy using ephrinA1-loaded albumin microspheres
| العنوان: | Targeted lung cancer therapy using ephrinA1-loaded albumin microspheres |
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| المؤلفون: | Kamal A. Mohammed, Eugene P. Goldberg, Najmunnisa Nasreen, Hung-Yen Lee, Shriram Peruvemba |
| المصدر: | Journal of Pharmacy and Pharmacology. 63:1401-1410 |
| بيانات النشر: | Oxford University Press (OUP), 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Antimetabolites, Antineoplastic, Lung Neoplasms, Blotting, Western, Cell, Pharmaceutical Science, Focal adhesion, Mice, chemistry.chemical_compound, Drug Delivery Systems, Albumins, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, medicine, Animals, Humans, Molecular Targeted Therapy, Viability assay, Particle Size, Bovine serum albumin, Fluorescein isothiocyanate, Pharmacology, A549 cell, biology, Chemistry, Albumin, Ephrin-A1, Molecular biology, Microspheres, medicine.anatomical_structure, Cell culture, biology.protein, Cattle |
| الوصف: | Objectives EphrinA1, the ligand of EphA2 receptor tyrosine kinase, has been proven to suppress the growth of tumours. The aim of this study was to conjugate ephrinA1 on the surface of albumin microspheres and investigate the non-small cell lung carcinoma growth and migration in vitro. Methods Bovine serum albumin microspheres were designed and synthesized using a natural polymer albumin by emulsification chemical cross-linking. EphrinA1 was then conjugated on the surface of microspheres by imine formation. The microspheres conjugated with ephrinA1 (ephrinA1-MS) were characterized for particle size, surface morphology, loading efficiency and stability in vitro. The ephrinA1-MS were labelled with fluorescein isothiocyanate to determine phagocytosis. In addition, the effects of ephrinA1-MS on A549 cell growth and migration were determined. Key findings Albumin microspheres exhibited low toxicity for A549 cells (above 90% cell viability). More than 80% of microspheres were phagocytosed within 2 h of incubation. EphrinA1-MS decreased the expression of focal adhesion kinase more effectively than recombinant ephrinA1 alone. Furthermore, ephrinA1-MS showed significant inhibition of non-small cell lung cancer migration when compared with resting cells. EphrinA1-MS attenuated the growth of tumour colonies in matrigels. Conclusions The developed ephrinA1-MS may serve as potential carriers for targeted delivery of the tumour suppressive protein ephrinA1, with minimal cytotoxic effects and greater antitumour therapeutic efficacy against non-small cell lung cancer. |
| تدمد: | 2042-7158 0022-3573 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfab1c8ac7944dff106c7c557f857ce6 https://doi.org/10.1111/j.2042-7158.2011.01306.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bfab1c8ac7944dff106c7c557f857ce6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20427158 00223573 |
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