Perspectives from Ten Years of Protist Studies by High‐Throughput Metabarcoding
| العنوان: | Perspectives from Ten Years of Protist Studies by High‐Throughput Metabarcoding |
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| المؤلفون: | George B. McManus, Luciana F. Santoferrara, Sabine Filker, Ramiro Logares, Micah Dunthorn, Fabien Burki |
| المساهمون: | National Science Foundation (US), Science for Life Laboratory, Agencia Estatal de Investigación (España) |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Eukaryota, High-Throughput Nucleotide Sequencing, Protist, Biodiversity, 030108 mycology & parasitology, Biology, medicine.disease_cause, Microbiology, Ecological patterns, Phylogenetic, 03 medical and health sciences, 030104 developmental biology, Biogeography, Evolutionary biology, medicine, DNA Barcoding, Taxonomic, Biological interactions, Function, Biologie, Throughput (business), Phylogeny |
| الوصف: | 13 pages, 3 figures.-- This article resulted from the symposium “Ten Years of Metabarcoding: What Have we Learned and How Do we Move Forward?” held during the VIII European Congress of Protistology-ISOP Joint Meeting, Rome, Italy, in July 2019.-- This is the pre-peer reviewed version of the following article: L.F. Santoferrara, F. Burki, S. Filker, R. Logares, M. Dunthorn, G. McManus, Perspectives from ten years of protist studies by high-throughput metabarcoding, Journal of Eukaryotic Microbiology 67(5):612-622(2020), which has been published in final form at https://doi.org/10.1111/jeu.12813. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions During the last decade, high-throughput metabarcoding became routine for analyzing protistan diversity and distributions in nature. Amid a multitude of exciting findings, scientists have also identified and addressed technical and biological limitations, although problems still exist for inference of meaningful taxonomic and ecological knowledge based on short DNA sequences. Given the extensive use of this approach, it is critical to settle our understanding on its strengths and weaknesses and to synthesize up-to-date methodological and conceptual trends. This article summarizes key scientific and technical findings, and identifies current and future directions in protist research that uses metabarcoding Additional support for this work includes a grant by the US National Science Foundation (OCE1924527) to GM and LS, and a fellowship from the Science for Life Laboratory (SciLifeLab) to FB With the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI) |
| تدمد: | 1550-7408 1066-5234 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfac1b8da83aaa12f07002521fa77b7d https://doi.org/10.1111/jeu.12813 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bfac1b8da83aaa12f07002521fa77b7d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15507408 10665234 |
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