Two Plastid Fatty Acid Exporters Contribute to Seed Oil Accumulation in Arabidopsis
| العنوان: | Two Plastid Fatty Acid Exporters Contribute to Seed Oil Accumulation in Arabidopsis |
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| المؤلفون: | Qin Song, Keming Luo, Xiaohong Li, Qing Li, Jianru Zuo, Hanwen Li, Aihui Liu, Shufeng Wang, Shengting Li, Nannan Li, Xin Zhao, Hongjun Meng, Liang Guo, Zhen Zhang |
| المصدر: | Plant Physiol |
| بيانات النشر: | Oxford University Press (OUP), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, food.ingredient, Physiology, Arabidopsis, Plant Science, 01 natural sciences, Chloroplast membrane, chemistry.chemical_compound, food, Biosynthesis, Genetics, Arabidopsis thaliana, Plastids, Plastid, Research Articles, chemistry.chemical_classification, biology, Endoplasmic reticulum, Fatty Acids, fungi, food and beverages, Fatty acid, biology.organism_classification, chemistry, Biochemistry, Seeds, Cotyledon, 010606 plant biology & botany |
| الوصف: | Triacylglycerols (TAGs) are the major storage form of seed oil in oilseed plants. They are biosynthesized de novo in seed plastids and then transported into the endoplasmic reticulum. However, the transport mechanism for plastid fatty acids in developing seeds remains unknown. Here, we isolated two novel plastid fatty acid exporters (FATTYACID EXPORT 2 [FAX2] and FAX4, respectively) specifically abundant in seed embryos during the seed-filling stage in Arabidopsis (Arabidopsis thaliana). FAX2 and FAX4 were both localized to the chloroplast membrane. FAX2 and FAX4 loss-of-function mutations caused deficiencies in embryo and cotyledon development. Seeds of fax2fax4 double mutants exhibited significantly reduced TAG contents but elevated levels of plastid lipid contents compared with those of wild-type plants. By contrast, overexpression of FAX2 or FAX4 enhanced TAG deposition. Seed-feeding experiments showed that the two FAX proteins transported (14)C-plastid fatty acids and (13)C-oleic acids for TAG biosynthesis during the seed-filling stage. Together, our data demonstrate that FAX2 and FAX4 play critical roles in transporting plastid fatty acids for TAG biosynthesis during seed embryo development. These two transporters may have broad application for increasing oil yield in oilseed crops. |
| تدمد: | 1532-2548 0032-0889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfee4d0c6ae9ff9dd1cadf240f86bc7c https://doi.org/10.1104/pp.19.01344 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....bfee4d0c6ae9ff9dd1cadf240f86bc7c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
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