Promoter exchange of the cryptic nonribosomal peptide synthetase gene for oligopeptide production in Aspergillus oryzae
| العنوان: | Promoter exchange of the cryptic nonribosomal peptide synthetase gene for oligopeptide production in Aspergillus oryzae |
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| المؤلفون: | Kobkul Laoteng, Jutamas Anantayanon, Chanikul Chutrakul, Sukanya Jeennor, Sarocha Panchanawaporn |
| المصدر: | Journal of Microbiology. 60:47-56 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Aspergillus oryzae, viruses, ACV synthetase, Applied Microbiology and Biotechnology, Microbiology, Fungal Proteins, Nonribosomal peptide, Gene Expression Regulation, Fungal, Peptide Synthases, Promoter Regions, Genetic, skin and connective tissue diseases, Magnesium ion, Gene, chemistry.chemical_classification, Oligopeptide, biology, virus diseases, Promoter, General Medicine, biology.organism_classification, Biochemistry, chemistry, Biosynthetic process, biology.protein, Oligopeptides |
| الوصف: | Oligopeptides with functional activities are of current interest in the nutraceutical and medical sectors. The development of the biosynthetic process of oligopeptides through a nonribosomal peptide synthetase (NRPS) system has become more challenging. To develop a production platform for nonribosomal peptides (NRPs), reprogramming of transcriptional regulation of the acv gene encoded ACV synthetase (ACVS) was implemented in Aspergillus oryzae using the CRISPR-Cas9 system. Awakening silent acv expression was successfully achieved by promoter substitution. Among the three exchanged promoters, AoPgpdA, AoPtef1, and PtPtoxA, the replacement of the native promoter with AoPgpdA led to the highest ACV production in A. oryzae. However, the ACV production of the AoPGpdA strain was also dependent on the medium composition, in which urea was the best nitrogen source, and a C:N ratio of 20:1 was optimal for tripeptide production. In addition to cell growth, magnesium ions are an essential element for ACV production and might participate in ACVS activity. It was also found that ACV was the growth-associated product of the engineered strain that might be a result of constitutive transcriptional control by the AoPgpdA promoter. This study offers a potential strategy for nonribosomal ACV production using the fungal system, which is applicable for redesigning bioactive oligopeptides with industrial relevance. |
| تدمد: | 1976-3794 1225-8873 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c02d42a4b6236de6ca77b3e284df0a9a https://doi.org/10.1007/s12275-022-1442-3 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....c02d42a4b6236de6ca77b3e284df0a9a |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 19763794 12258873 |
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