Circadian gene expression in mouse renal proximal tubule
| العنوان: | Circadian gene expression in mouse renal proximal tubule |
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| المؤلفون: | Molly A. Bingham, Kim Neijman, Chin-Rang Yang, Angel Aponte, Angela Mak, Hiroaki Kikuchi, Hyun Jun Jung, Brian G. Poll, Viswanathan Raghuram, Euijung Park, Chung-Lin Chou, Lihe Chen, Jens Leipziger, Mark A. Knepper, Margo Dona |
| المصدر: | Bingham, M A, Neijman, K, Yang, C R, Aponte, A, Mak, A, Kikuchi, H, Jung, H J, Poll, B G, Raghuram, V, Park, E, Chou, C L, Chen, L, Leipziger, J, Knepper, M A & Dona, M 2023, ' Circadian gene expression in mouse renal proximal tubule ', American Journal of Physiology: Renal Physiology, vol. 324, no. 3, pp. F301-F314 . https://doi.org/10.1152/ajprenal.00231.2022 American Journal of Physiology : Renal Physiology, 324, F301-F314 American Journal of Physiology : Renal Physiology, 324, 3, pp. F301-F314 |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | transcriptomics, All institutes and research themes of the Radboud University Medical Center, proteomics, Physiology, proximal tubule, Vascular damage Radboud Institute for Molecular Life Sciences [Radboudumc 16], RNA sequencing, circadian variation |
| الوصف: | Item does not contain fulltext Circadian variability in kidney function is well recognized but is often ignored as a potential confounding variable in physiological experiments. Here, we have created a data resource consisting of expression levels for mRNA transcripts in microdissected proximal tubule segments from mice as a function of the time of day. Small-sample RNA sequencing was applied to microdissected S1 proximal convoluted tubules and S2 proximal straight tubules. After stringent filtering, the data were analyzed using JTK-Cycle to detect periodicity. The data set is provided as a user-friendly webpage at https://esbl.nhlbi.nih.gov/Databases/Circadian-Prox2/. In proximal convoluted tubules, 234 transcripts varied in a circadian manner (4.0% of the total). In proximal straight tubules, 334 transcripts varied in a circadian manner (5.3%). Transcripts previously known to be associated with corticosteroid action and with increased flow were found to be overrepresented among circadian transcripts peaking during the "dark" portion of the day [zeitgeber time (ZT)14-22], corresponding to peak levels of corticosterone and glomerular filtration rate in mice. To ask whether there is a time-of-day dependence of protein abundances in the kidney, we carried out LC-MS/MS-based proteomics in whole mouse kidneys at ZT12 and ZT0. The full data set (n = 6,546 proteins) is available at https://esbl.nhlbi.nih.gov/Databases/Circadian-Proteome/. Overall, 293 proteins were differentially expressed between ZT12 and ZT0 (197 proteins greater at ZT12 and 96 proteins greater at ZT0). Among the regulated proteins, only nine proteins were found to be periodic in the RNA-sequencing analysis, suggesting a high level of posttranscriptional regulation of protein abundances.NEW & NOTEWORTHY Circadian variation in gene expression can be an important determinant in the regulation of kidney function. The authors used RNA-sequencing transcriptomics and LC-MS/MS-based proteomics to identify gene products expressed in a periodic manner. The data were used to construct user-friendly web resources. |
| تدمد: | 1931-857X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c037b3699b9ffbec9a72178fa392149c https://hdl.handle.net/2066/290755 |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....c037b3699b9ffbec9a72178fa392149c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1931857X |
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