Suppression of the NF‑κB signaling pathway in colon cancer cells by the natural compound Riccardin D from Dumortierahirsute
| العنوان: | Suppression of the NF‑κB signaling pathway in colon cancer cells by the natural compound Riccardin D from Dumortierahirsute |
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| المؤلفون: | Huiping Liu, Yun Luan, Fang Chen, Guoning Li, Rongmei Wang, Bin Zhang, Feng Kong, Wen Jiang, Xia Xue, Deqing Sun, Jing Wu |
| المصدر: | Molecular Medicine Reports |
| بيانات النشر: | Spandidos Publications, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Hepatophyta, Models, Molecular, 0301 basic medicine, Cancer Research, Cell Membrane Permeability, Membrane permeability, Riccardin D, Cell, Molecular Conformation, Gene Expression, Apoptosis, colorectal cancer, nuclear factor-κB signaling, Biochemistry, Structure-Activity Relationship, 03 medical and health sciences, 0302 clinical medicine, Genes, Reporter, Cell Line, Tumor, autodock analysis, Stilbenes, Genetics, medicine, Humans, Molecular Biology, Cell Proliferation, Biological Products, Oncogene, Plant Extracts, Chemistry, Cell growth, Phenyl Ethers, NF-kappa B, Articles, Cell cycle, Mitochondria, 030104 developmental biology, medicine.anatomical_structure, Oncology, Cyclooxygenase 2, 030220 oncology & carcinogenesis, Colonic Neoplasms, Cancer research, Molecular Medicine, Tumor necrosis factor alpha, Signal transduction, Protein Binding, Signal Transduction |
| الوصف: | Colorectal cancer (CRC) is a major cause of mortality and morbidity. Chronic inflammation is closely associated with the development, progression and prognosis of the majority of intestinal malignancies. In recent years, targeting the nuclear factor (NF)-κB signaling pathway for CRC therapy has become an attractive strategy. Riccardin D, a novel macrocyclicbis (bibenzyl) compound, was isolated from the Chinese liverwort plant. Previous studies have suggested that Riccardin D exerted chemo-preventative effects against the intestinal malignancy formation. In the present study, cell counting kit-8, Hochest 33258 staining, mitochondria membrane permeability assay, western blotting analysis, reverse transcription-polymerase chain reaction, luciferase reporter gene assay and molecular modeling analysis were performed to detect the effect and mechanisms of Riccardin D on human colon cancer cells. The results demonstrated that Riccardin D significantly inhibited the growth of HT-29 cells. In addition, the cDNA expression of cyclooxygenase-2, and the protein expression and activity of NF-κB and tumor necrosis factor-α were downregulated; however, the protein expression of cleaved caspase-3 and −9, and cleaved poly (adenosine diphosphate-ribose) polymerase, and the B-cell lymphoma (Bcl)-2: Bcl-2-associated X protein ratio were upregulated. Furthermore, Auto Dock analysis identified binding sites between Riccardin D and NF-κB. These results indicated that Riccardin D may inhibit cell proliferation and induce apoptosis in HT-29 cells, which may be associated with the blocking of the NF-κB signaling pathway. Thus, Riccardin D should be investigated as an NF-κB inhibitor in cancer therapy. |
| تدمد: | 1791-3004 1791-2997 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0d90d8de62432245c69b0f057ca1d93 https://doi.org/10.3892/mmr.2018.8617 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c0d90d8de62432245c69b0f057ca1d93 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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