Gene expression profiling identifies potential molecular markers of papillary thyroid carcinoma
| العنوان: | Gene expression profiling identifies potential molecular markers of papillary thyroid carcinoma |
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| المؤلفون: | Robert Suriano, Anthony Policastro, Codrin Iacob, Niradiz Reyes, Nina Suslina, Raj K. Tiwari, Ismael Reyes, Augustine Moscatello, Stimson P. Schantz, Jan Geliebter |
| المصدر: | Cancer Biomarkers. 24:71-83 |
| بيانات النشر: | IOS Press, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Male, Cancer Research, KLK10, Biology, Syndecan 1, Transcriptome, Young Adult, Biomarkers, Tumor, Genetics, medicine, Humans, 0501 psychology and cognitive sciences, Thyroid Neoplasms, Thyroid cancer, Aged, Neoplasm Staging, 0505 law, Regulation of gene expression, Microarray analysis techniques, Gene Expression Profiling, 05 social sciences, General Medicine, Middle Aged, medicine.disease, Gene Expression Regulation, Neoplastic, Gene expression profiling, Oncology, Thyroid Cancer, Papillary, 050501 criminology, Cancer research, Female, DNA microarray, 050104 developmental & child psychology |
| الوصف: | Background Thyroid cancer is the most common endocrine malignancy worldwide, with the predominant form papillary thyroid carcinoma (PTC) representing approximately 80% of cases. Objective This study was addressed to identify potential genes and pathways involved in the pathogenesis of PTC and potential novel biomarkers for this disease. Methods Gene expression profiling was carried out by DNA microarray technology. Validation of microarray data by qRT-PCR, western blot, and enzyme linked immunosorbent assay was also performed in a selected set of genes and gene products, with the potential to be used as diagnostic or prognostic biomarkers, such as those associated with cell adhesion, extracellular matrix (ECM) remodeling and immune/inflammatory response. Results In this study we found that upregulation of extracellular activities, such as proteoglycans, ECM-receptor interaction, and cell adhesion molecules, were the most prominent feature of PTC. Significantly over-expressed genes included SDC1 (syndecan 1), SDC4 (syndecan 4), KLK7 (kallikrein-related peptidase 7), KLK10 (kallikrein-related peptidase 10), SLPI (secretory leukocyte peptidase inhibitor), GDF15 (growth/differentiation factor-15), ALOX5 (arachidonate 5-lipoxygenase), SFRP2 (secreted Frizzled-related protein 2), among others. Further, elevated KLK10 levels were detected in patients with PTC. Many of these genes belong to KEGG pathway "Proteoglycans in cancer". Conclusions Using DNA microarray analysis allowed the identification of genes and pathways with known important roles in malignant transformation, and also the discovery of novel genes that may be potential biomarkers for PTC. |
| تدمد: | 1875-8592 1574-0153 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c10bf26876f1fb29846db29cb7737899 https://doi.org/10.3233/cbm-181758 |
| رقم الأكسشن: | edsair.doi.dedup.....c10bf26876f1fb29846db29cb7737899 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18758592 15740153 |
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