Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing
العنوان: | Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing |
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المؤلفون: | Gulen Yesilbag Tonga, Yi-Wei Lee, Vincent M. Rotello, Rubul Mout, Moumita Ray, Kanae Sasaki, Tristan Tay |
المصدر: | ACS Nano. 11:2452-2458 |
بيانات النشر: | American Chemical Society (ACS), 2017. |
سنة النشر: | 2017 |
مصطلحات موضوعية: | 0301 basic medicine, General Physics and Astronomy, 02 engineering and technology, Computational biology, Gene delivery, Biology, Protein Engineering, Article, Genome engineering, 03 medical and health sciences, Cytosol, Genome editing, CRISPR, General Materials Science, Guide RNA, Gene Editing, Genetics, CRISPR interference, Cas9, Gene Transfer Techniques, General Engineering, Gene targeting, 021001 nanoscience & nanotechnology, 030104 developmental biology, Ribonucleoproteins, CRISPR-Cas Systems, 0210 nano-technology |
الوصف: | Genome editing through the delivery of CRISPR/Cas9-ribonulceoprotein (Cas9-RNP) reduces unwanted gene targeting and avoids integrational mutagenesis that can occur through gene delivery strategies. Direct and efficient delivery of Cas9-RNP into the cytosol followed by translocation to the nucleus remains a challenge. Here we report a remarkably high efficient (~90%) direct cytoplasmic/nuclear delivery of Cas9 protein complexed with a guide RNA (sgRNA) through the co-engineering of Cas9 protein and carrier nanoparticles. This construct provides effective (~30%) gene editing efficiency and opens up new opportunities in studying genome dynamics. |
تدمد: | 1936-086X 1936-0851 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c226d98a3220a398655d2ef693756451 https://doi.org/10.1021/acsnano.6b07600 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....c226d98a3220a398655d2ef693756451 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 1936086X 19360851 |
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