Involvement of GLUT1 and GLUT3 in the growth of canine melanoma cells
| العنوان: | Involvement of GLUT1 and GLUT3 in the growth of canine melanoma cells |
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| المؤلفون: | Tomohiro Nakayama, Yoko Suwabe, Naoya Yachiku, Mana Sugimura, Shinichi Namba, Manami Kuji, Hiroshi Sugiya, Taku Kitanaka, Tadayoshi Konno, Rei Nakano, Nanako Kitanaka |
| المصدر: | PLoS ONE PLoS ONE, Vol 16, Iss 2, p e0243859 (2021) |
| بيانات النشر: | Public Library of Science, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Skin Neoplasms, Physiology, Glucose uptake, Biochemistry, 0302 clinical medicine, Glucose Metabolism, Medicine and Health Sciences, Melanoma, Skin Tumors, Cultured Tumor Cells, Glucose Transporter Type 1, Multidisciplinary, biology, Glucose Transporter Type 3, Chemistry, Organic Compounds, Monosaccharides, Glucose analog, Small interfering RNA, Nucleic acids, Oncology, 030220 oncology & carcinogenesis, Physical Sciences, Medicine, Melanoma Cells, Carbohydrate Metabolism, Biological Cultures, Research Article, medicine.medical_specialty, Science, Carbohydrates, Dermatology, Research and Analysis Methods, Transfection, 03 medical and health sciences, Dogs, Internal medicine, Cell Line, Tumor, medicine, Canine Melanoma, Genetics, Animals, Lactic Acid, RNA, Messenger, Non-coding RNA, Molecular Biology Techniques, Molecular Biology, Secretion, Cell Proliferation, Biology and life sciences, Cell growth, Organic Chemistry, Glucose transporter, Chemical Compounds, Cancers and Neoplasms, Cell Cultures, Gene regulation, 030104 developmental biology, Endocrinology, Glucose, Metabolism, Cancer cell, biology.protein, RNA, GLUT1, Gene expression, Physiological Processes, GLUT3 |
| الوصف: | The rate of glucose uptake dramatically increases in cancer cells even in the presence of oxygen and fully functioning mitochondria. Cancer cells produce ATP by glycolysis rather than oxidative phosphorylation under aerobic conditions, a process termed as the “Warburg effect.” In the present study, we treated canine melanoma cells with the glucose analog 2-deoxy-D-glucose (2-DG) and investigated its effect on cell growth. 2-DG attenuated cell growth in a time- and dose-dependent manner. Cell growth was also inhibited following treatment with the glucose transporter (GLUT) inhibitor WZB-117. The treatment of 2-DG and WZB-117 attenuated the glucose consumption, lactate secretion and glucose uptake of the cells. The mRNA expression of the subtypes of GLUT was examined and GLUT1 and GLUT3 were found to be expressed in melanoma cells. The growth, glucose consumption and lactate secretion of melanoma cells transfected with siRNAs of specific for GLUT1 and GLUT3 was suppressed. These findings suggest that glucose uptake via GLUT1 and GLUT3 plays a crucial role for the growth of canine melanoma cells. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2473dfc8d2ac1784425630d081d48ce http://europepmc.org/articles/PMC7861381 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c2473dfc8d2ac1784425630d081d48ce |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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