Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A
| العنوان: | Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A |
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| المؤلفون: | Wen-Pu Shi, Xi-Wen Dong, Lin Yuan, Zhao Zhang, Huijie Bian, Jia-Yue Li, Jian Cui, Zhi-Nan Chen, Zheng Zhang, Dan Luo, Ziling Wang, Peng Lin |
| المصدر: | Journal of Immunology Research, Vol 2018 (2018) Journal of Immunology Research |
| بيانات النشر: | Hindawi Limited, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, lcsh:Immunologic diseases. Allergy, Lung Neoplasms, Article Subject, Immunology, Down-Regulation, Mice, Nude, Biology, Metastasis, 03 medical and health sciences, Mice, 0302 clinical medicine, Downregulation and upregulation, In vivo, Cell Movement, Carcinoma, Non-Small-Cell Lung, microRNA, Carcinoma, medicine, Immunology and Allergy, Animals, Humans, Neoplasm Metastasis, Lung cancer, Cell Proliferation, Mice, Inbred BALB C, Cell growth, HEK 293 cells, Semaphorin-3A, General Medicine, Neoplasms, Experimental, medicine.disease, respiratory tract diseases, MicroRNAs, 030104 developmental biology, HEK293 Cells, 030220 oncology & carcinogenesis, Cancer research, Female, lcsh:RC581-607, Research Article |
| الوصف: | miR-362 is a recently discovered member of the microRNA family, and it modulates a variety of physical activities and plays an important role in the occurrence and development of many tumors. However, the biological functions of hsa-miR-362-5p in non-small-cell lung carcinoma (NSCLC) are unknown. Transwell assay and colony formation were used to determine the migration, invasion, and proliferation of NSCLC cells in vitro. A subcutaneous tumor model in nude mice was established to detect NSCLC tumor growth in vivo. The direct binding of miR-362 to the 3′UTR of Semaphorin 3A (Sema3A) was confirmed by luciferase reporter assay. In this study, we found that the level of miR-362 was higher in NSCLC tissues than in adjacent normal tissues and that the level of miR-362 expression was also elevated in five NSCLC cell lines (A549, 95-D, H1299, H292, and H460) relative to a human normal lung epithelial cell line (BEAS2B). Furthermore, miR-362 promoted NSCLC cell invasion, migration, and colony formation in vitro and tumor formation in vivo. Next, we identified the miR-362 target gene Sema3A, which is significantly correlated with metastasis. Sema3A expression was increased in normal tissues relative to NSCLC tissues. This result is consistent with the fact that miR-362 expression is negatively correlated with Sema3A expression in clinical tissue samples and indicated that miR-362 can regulate Sema3A expression in NSCLC cells and consequently affect NSCLC invasion, migration, and colony formation. Taken together, these findings on the newly identified miR-362/Sema3A axis elucidate the molecular mechanism of NSCLC invasion and migration and could lead to a potential therapeutic target in NSCLC treatment. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 2314-7156 2314-8861 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2728dd67e61dedf7b3030a507a7dc64 https://doaj.org/article/79df04f963f14bc482f9c338b4ef5b20 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c2728dd67e61dedf7b3030a507a7dc64 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23147156 23148861 |
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