Africa-wide evaluation of host biomarkers in QuantiFERON supernatants for the diagnosis of pulmonary tuberculosis
| العنوان: | Africa-wide evaluation of host biomarkers in QuantiFERON supernatants for the diagnosis of pulmonary tuberculosis |
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| المؤلفون: | Chegou, N.N., Sutherland, J.S., Namuganga, A.R., Corstjens, P.L.A.M., Geluk, A., Gebremichael, G., Mendy, J., Malherbe, S., Stanley, K., Spuy, G.D. van der, Kriel, M., Loxton, A.G., Kriel, B., Simukonda, F., Bekele, Y., Sheehama, J.A., Nelongo, J., Vyver, M. van der, Gebrexabher, A., Hailu, H., Esterhuyse, M.M., Rosenkrands, I., Aagard, C., Kidd, M., Kassa, D., Mihret, A., Howe, R., Cliff, J.M., Crampin, A.C., Mayanja-Kizza, H., Kaufmann, S.H.E., Dockrell, H.M., Ottenhoff, T.H.M., Walzl, G., AE-TBC Consortium |
| المصدر: | Scientific Reports, 8 Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, Vascular Endothelial Growth Factor A, medicine.medical_specialty, Tuberculosis, VEGF receptors, lcsh:Medicine, HIV Infections, Disease, Sensitivity and Specificity, Article, QuantiFERON, 03 medical and health sciences, Interferon-gamma, 0302 clinical medicine, Pulmonary tuberculosis, Internal medicine, Active tb, medicine, Humans, Mass Screening, lcsh:Science, Chemokine CCL4, Tuberculosis, Pulmonary, Multidisciplinary, biology, business.industry, lcsh:R, Middle Aged, Transforming Growth Factor alpha, medicine.disease, 030104 developmental biology, 030228 respiratory system, Uncertain diagnosis, Africa, biology.protein, Cytokines, lcsh:Q, Female, Hiv status, business, Biomarkers |
| الوصف: | We investigated host-derived biomarkers that were previously identified in QuantiFERON supernatants, in a large pan-African study. We recruited individuals presenting with symptoms of pulmonary TB at seven peripheral healthcare facilities in six African countries, prior to assessment for TB disease. We then evaluated the concentrations of 12 biomarkers in stored QuantiFERON supernatants using the Luminex platform. Based on laboratory, clinical and radiological findings and a pre-established algorithm, participants were classified as TB disease or other respiratory diseases(ORD). Of the 514 individuals included in the study, 179(34.8%) had TB disease, 274(51.5%) had ORD and 61(11.5%) had an uncertain diagnosis. A biosignature comprising unstimulated IFN-γ, MIP-1β, TGF-α and antigen-specific levels of TGF-α and VEGF, identified on a training sample set (n = 311), validated by diagnosing TB disease in the test set (n = 134) with an AUC of 0.81(95% CI, 0.76–0.86), corresponding to a sensitivity of 64.2%(95% CI, 49.7–76.5%) and specificity of 82.7%(95% CI, 72.4–89.9%). Host biomarkers detected in QuantiFERON supernatants can contribute to the diagnosis of active TB disease amongst people presenting with symptoms requiring investigation for TB disease, regardless of HIV status or ethnicity in Africa. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2c81504154aeae4f9266eafc61061f7 https://doi.org/10.1038/s41598-018-20855-7 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c2c81504154aeae4f9266eafc61061f7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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