Spongy Polyethersulfone Membrane for Hepatocyte Cultivation: Studies on Human Hepatoma C3A Cells
العنوان: | Spongy Polyethersulfone Membrane for Hepatocyte Cultivation: Studies on Human Hepatoma C3A Cells |
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المؤلفون: | Anna Śmietanka, Konrad Dudziński, Barbara Gajkowska, Andrzej Chwojnowski, Andrzej Werynski, A. Kinasiewicz |
المصدر: | Artificial Organs. 32:747-752 |
بيانات النشر: | Wiley, 2008. |
سنة النشر: | 2008 |
مصطلحات موضوعية: | Carcinoma, Hepatocellular, Cell Survival, Polymers, Cell Culture Techniques, Biomedical Engineering, Medicine (miscellaneous), Bioengineering, Biomaterials, Rhodamine, Mice, chemistry.chemical_compound, Cell Line, Tumor, Cell Adhesion, Fluorescence microscope, medicine, Animals, Humans, Sulfones, Viability assay, Cells, Cultured, Macrophages, Bile Canaliculi, Membranes, Artificial, General Medicine, In vitro, Staining, Membrane, medicine.anatomical_structure, Biochemistry, chemistry, Cell culture, Hepatocyte, Hepatocytes, Biophysics |
الوصف: | There are different types of membranes used for hepatocyte cultivation. In our studies, spongy polyethersulfone (PES) membranes were examined as a support for hepatic cell cultivation in vitro. The extended surface of the membranes allows to introduce a high cell number especially in three-dimensional gel structure. Scanning electron microscopy analysis indicated that C3A cells used in our experiments grew well on PES membranes forming microvilli characteristic for normal hepatocytes. Analysis of cell viability proved that spongy PES membrane is well tolerated by J774 macrophages and did not stimulate nitric oxide synthesis. Bile canalicular structures were observed in fluorescence microscopy after F-actin staining with tetramethyl rhodamine iso-thiocyanate (TRITC)-phalloidin. The C3A cells showed high affinity to the PES membranes and adhered to almost 90% during the initial 24 h of incubation. Albumin production increased during static culture from the value of 805.2 +/- 284.4 (ng/24 h/initial 10(6) cells) during the first days, to 2017.6 +/- 505.9 (ng/24 h/initial 10(6) cells) after 10 days of culture. In conclusion, the spongy PES membranes can be used as scaffold for hepatocyte cultivation, especially for the creation of three-dimensional environments. |
تدمد: | 1525-1594 0160-564X |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c301872f3e2d46dbfe7217d87e43b643 https://doi.org/10.1111/j.1525-1594.2008.00600.x |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi.dedup.....c301872f3e2d46dbfe7217d87e43b643 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 15251594 0160564X |
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