The Impact of Melatonin and NLRP3 Inflammasome on the Expression of microRNAs in Aged Muscle
| العنوان: | The Impact of Melatonin and NLRP3 Inflammasome on the Expression of microRNAs in Aged Muscle |
|---|---|
| المؤلفون: | Marisol Fernández-Ortiz, Ramy K. A. Sayed, Tomás de Haro, Darío Acuña-Castroviejo, Iryna Rusanova, César Rodríguez-Santana, Paula Aranda Martínez, Ana Guerra-Librero, José Fernández-Martínez, Germaine Escames |
| المساهمون: | [Sayed,RK] Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Sohag University, Sohag. [Sayed,RK, Fernández-Ortiz,M, Fernández-Martínez,J, Aranda Martínez,P, Guerra-Librero,A, Rodríguez-Santana,C, Escames,G, Acuña-Castroviejo,D, Rusanova,I] Centro de Investigación Biomédica, Departamento de Fisiología, Facultad de Medicina, Instituto de Biotecnología, Parque Tecnológico de Ciencias de la Salud, Universidad de Granada, Granada, Spain. [de Haro,T, Acuña-Castroviejo,D] UGC de Laboratorios Clínicos, Hospital Universitario San Cecilio, Granada, Spain. [Escames,G, Rusanova,I] CIBERfes, Ibs. Granada, Granada, Spain. [Rusanova,I] Department of Biochemistry and Molecular Biology I, Faculty of Science, University of Granada, Granada, Spain., This study was partially supported by grants from the Instituto de Salud Carlos III (Ministerio de Economía y Competitividad, Spain), through the projects, PI16-00519, PI19-01372, and CB16-10-00238 (Co-funded by European Regional Development Fund/European Social Fund) 'Investing in your future'), and from the Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía (CTS-101), Spain. M.F.-O. and J.F.-M. are supported by FPU fellowships from the Ministerio de Educación, Spain |
| المصدر: | Antioxidants Volume 10 Issue 4 Antioxidants, Vol 10, Iss 524, p 524 (2021) Digibug: Repositorio Institucional de la Universidad de Granada Universidad de Granada (UGR) Digibug. Repositorio Institucional de la Universidad de Granada instname |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Aging, Physiology, Clinical Biochemistry, Mutant, bcl-2, Envejecimiento, melatonin, Biochemistry, Melatonina, 0302 clinical medicine, Anatomy::Tissues::Muscles::Muscle, Striated::Muscle, Skeletal::Muscle Fibers, Skeletal [Medical Subject Headings], Organisms::Eukaryota::Animals [Medical Subject Headings], Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Cytokines::Interleukins::Interleukin-1 [Medical Subject Headings], NF-kB, Melatonin, MicroARNs, Inflammasome, Anatomy::Musculoskeletal System::Muscles::Muscle, Skeletal [Medical Subject Headings], Pro-caspase-1, microRNAs, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Neoplasm Proteins::Tumor Suppressor Proteins::Tumor Suppressor Protein p53 [Medical Subject Headings], Muscle fibers, medicine.anatomical_structure, Caspase-3, 030220 oncology & carcinogenesis, Technology and Food and Beverages::Food and Beverages::Food::Dietary Supplements [Medical Subject Headings], NLRP3-inflammasome, Collagen, medicine.symptom, medicine.drug, medicine.medical_specialty, void(null), Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Scleroproteins::Extracellular Matrix Proteins::Collagen [Medical Subject javascript], Inflammation, Biology, Article, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::NF-kappa B [Medical Subject Headings], 03 medical and health sciences, Gastrocnemius muscle, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Intracellular Signaling Peptides and Proteins::Apoptosis Regulatory Proteins::Proto-Oncogene Proteins c-bcl-2::bcl-2-Associated X Protein [Medical Subject Headings], Internal medicine, microRNA, medicine, Caspasa 3, Caspasa 1, Molecular Biology, Phenomena and Processes::Biological Phenomena [Medical Subject Headings], Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings], Fibras Musculares Esqueléticas, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intracellular Signaling Peptides and Proteins::Apoptosis Regulatory Proteins::Caspases::Caspases, Effector::Caspase 3 [Medical Subject Headings], lcsh:RM1-950, aging, Wild type, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intracellular Signaling Peptides and Proteins::Apoptosis Regulatory Proteins::Caspases::Caspases, Initiator::Caspase 1 [Medical Subject Headings], Skeletal muscle, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction [Medical Subject Headings], Cell Biology, Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, Antisense::MicroRNAs [Medical Subject Headings], NLRP3 inflammasome, FN-kappa B, MicroRNAs, lcsh:Therapeutics. Pharmacology, 030104 developmental biology, Endocrinology, Chemicals and Drugs::Macromolecular Substances::Multiprotein Complexes::Inflammasomes [Medical Subject Headings], NF-KB, Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Melatonin [Medical Subject Headings], Proteína con dominio pirina 3 de la familia NLR, Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Inflammation [Medical Subject Headings] |
| الوصف: | Muscular aging is a complex process and underlying physiological mechanisms are not fully clear. In recent years, the participation of the NF-kB pathway and the NLRP3 inflammasome in the chronic inflammation process that accompanies the skeletal muscle’s aging has been confirmed. microRNAs (miRs) form part of a gene regulatory machinery, and they control numerous biological processes including inflammatory pathways. In this work, we studied the expression of four miRs three of them are considered as inflammatory-related miRs (miR-21, miR-146a, and miR-223), and miR-483, which is related to the regulation of melatonin synthesis, among other targets. To investigate the changes of miRs expression in muscle along aging, the impact of inflammation, and the role of melatonin in aged skeletal muscle, we used the gastrocnemius muscle of wild type (WT) and NLRP3-knockout (NLRP3−) mice of 3, 12, and 24 months-old, with and without melatonin supplementation. The expression of miRs and pro-caspase-1, caspase-3, pro-IL-1β, bax, bcl-2, and p53, was investigated by qRT-PCR analysis. Histological examination of the gastrocnemius muscle was also done. The results showed that age increased the expression of miR-21 (p < 0.01), miR-146a, and miR-223 (p < 0.05, for both miRs) in WT mice, whereas the 24-months-old mutant mice revealed decline of miR-21 and miR-223 (p < 0.05), compared to WT age. The lack of NLRP3 inflammasome also improved the skeletal muscle fibers arrangement and reduced the collagen deposits compared with WT muscle during aging. For the first time, we showed that melatonin significantly reduced the expression of miR-21, miR-146a, and miR-223 (p < 0.05 for all ones, and p < 0.01 for miR-21 at 24 months old) in aged WT mice, increased miR-223 in NLRP3− mice (p < 0.05), and induced miR-483 expression in both mice strains, this increase being significant at 24 months of age. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c316b3032f8eaa45f1327991af6ad8c6 http://hdl.handle.net/10481/67940 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c316b3032f8eaa45f1327991af6ad8c6 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |