Killing of Alveolar Macrophages and of Monocytes that Have Responded to Granulocyte‐Macrophage Colony‐stimulating Factor by Human Lymphokine‐activated Killer Cells
| العنوان: | Killing of Alveolar Macrophages and of Monocytes that Have Responded to Granulocyte‐Macrophage Colony‐stimulating Factor by Human Lymphokine‐activated Killer Cells |
|---|---|
| المؤلفون: | Noriaki Inamura, Takeshi Ogura, Saburo Sone, Sukh Mahendra Singh, Hiroaki Yanagawa, Nakanishi M, Akio Okubo |
| المصدر: | Japanese Journal of Cancer Research : Gann |
| بيانات النشر: | Blackwell Publishing Ltd, 1989. |
| سنة النشر: | 1989 |
| مصطلحات موضوعية: | Cytotoxicity, Immunologic, Cancer Research, Colony‐stimulating factor, Biology, In Vitro Techniques, Peripheral blood mononuclear cell, Interleukin 2, Article, Monocytes, Biological Factors, Colony-Stimulating Factors, medicine, Macrophage, Humans, Growth Substances, Killer Cells, Lymphokine-Activated, Cells, Cultured, Interleukin 3, Immunity, Cellular, Lymphokine-activated killer cell, Monocyte, Macrophages, Lymphokine, Lymphokine‐activated killer cell, Granulocyte-Macrophage Colony-Stimulating Factor, Colony-stimulating factor, Molecular biology, Pulmonary Alveoli, Granulocyte macrophage colony-stimulating factor, medicine.anatomical_structure, Oncology, Immunology, Cytokines, Cell Division, medicine.drug |
| الوصف: | The susceptibilities of human blood monocytes and alveolar macrophages (AM) to cytotoxicity mediated by lymphokine (IL-l)-activated killer (LAK) cells were examined. Monocytes and AM of healthy donors were obtained by counter-flow centrifugal elutriation (CCE) and bronclioalvcolar lavage, respectively. The LAK activity induced by incubation of blood mononuclear cells (MNC) for 4 days with recombinant interleukin 2 (IL-2) was measured by a 4-h 51Cr release assay. The LAK cells were not cytotoxic to freshly isolated monocytes, but were cytotoxic to autologous fresh AM and monocytes that had been incubated for more than 4 days in medium alone. Blood monocytes that had been incubated for 4- days in medium with granulocyte-macrophage colony-stimulating factor (GM-CSF), macrophage colony-stimulating factor (M-CSF) or interleukin 3 (IL-3) were much more susceptible than untreated monocytes to the cytotoxicity of LAK cells. When blood monocytes were separated by CCE into subpopulations of three sizes (small, medium and large), the medium- and large-sized monocytes showed greater responses to GM-CSF in terms of DNA synthesis and colony formation than the small-sized cells. After treatment with GM-CSF for 4 days, these medium and large monocytes were more susceptible than the small monocytes to the cytotoxic action of LAK cells. These results suggest that LAK cells may be important in situ in down-regulating the functions of mature macrophages and blood monocytes that have responded to GM-CSF. |
| اللغة: | English |
| تدمد: | 1876-4673 0910-5050 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c34f37c56b9e1a11e443529ffae63f64 http://europepmc.org/articles/PMC5917807 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c34f37c56b9e1a11e443529ffae63f64 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18764673 09105050 |
|---|