A single point mutation causes one-way alteration of pheromone receptor function in two Heliothis species
| العنوان: | A single point mutation causes one-way alteration of pheromone receptor function in two Heliothis species |
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| المؤلفون: | Song Cao, Yang Liu, Bing Wang, Guirong Wang |
| المصدر: | iScience, Vol 24, Iss 9, Pp 102981-(2021) iScience |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Genetics, Mutation, Multidisciplinary, biology, Heliothis virescens, Ecology, Point mutation, Science, medicine.disease_cause, biology.organism_classification, Article, Heliothis subflexa, Biological sciences, Heliothis, Sex pheromone, medicine, Sensory neuroscience, Pheromone, Zoology, Function (biology), Neuroscience |
| الوصف: | Summary The sex pheromone processing system of moths has been a major focus of research on olfaction and speciation, as it is highly specific and closely related to reproductive isolation. The two noctuid moths Heliothis virescens and Heliothis subflexa have been used as a model for deciphering the mechanisms underlying differentiation in pheromone communication, but no information exist regarding the functions of the pheromone receptors (PRs) of H. subflexa. Here, we functionally characterized all candidate PRs of H. subflexa, and found that only the response profile of OR6 differed between the two species. Through domain swapping and site-directed mutation followed by functional characterization, we identified a critical amino acid in OR6 caused a one-way alteration in specificity. This result suggests HsubOR6 evolved from an ancestral OR6 gene with a HvirOR6-like function and implies that the evolutionary direction of the receptor specificity was from the H. virescens-like pattern to H. subflexa-like pattern. Graphical abstract Highlights • Six pheromone receptors (PRs) were functionally characterized in Heliothis subflexa • Function of one orthologous PR-OR6 differed between H. subflexa and H. virescens • A critical amino acid residue caused one-way alteration of OR6 specificity • This suggests HsubOR6 evolved from an ancestral gene with a HvirOR6-like function Ecology; Biological sciences; Zoology; Neuroscience; Sensory neuroscience |
| اللغة: | English |
| تدمد: | 2589-0042 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4295d6db377337d87442d16b024aedd http://www.sciencedirect.com/science/article/pii/S2589004221009494 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c4295d6db377337d87442d16b024aedd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 25890042 |
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