N-myc enhances the expression of a large set of genes functioning in ribosome biogenesis and protein synthesis
| العنوان: | N-myc enhances the expression of a large set of genes functioning in ribosome biogenesis and protein synthesis |
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| المؤلفون: | Linda J. Valentijn, Ilja Roobeek, P.A. Voûte, Peter van Sluis, Rogier Versteeg, Isabel Weis, Kathy Boon, Marie Christine Hermus, Huib N. Caron, Ronald van Asperen, Manfred Schwab |
| المساهمون: | Other departments |
| المصدر: | EMBO journal, 20(6), 1383-1393. Wiley-Blackwell |
| بيانات النشر: | Wiley, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Ribosomal Proteins, Time Factors, Genes, myc, Melanoma, Experimental, Ribosome biogenesis, Biology, Article, General Biochemistry, Genetics and Molecular Biology, Ribosome assembly, Neuroblastoma, Ribosomal protein, Humans, RNA, Messenger, Northern blot, Serial analysis of gene expression, Molecular Biology, Gene, Gene Library, General Immunology and Microbiology, Gene Expression Profiling, General Neuroscience, fungi, Ribosomal RNA, Molecular biology, Up-Regulation, Gene Expression Regulation, Neoplastic, RNA, Ribosomal, Protein Biosynthesis, Glycolysis, Ribosomes, N-Myc |
| الوصف: | The myc oncogenes are frequently activated in human tumors, but there is no comprehensive insight into the target genes and downstream cellular pathways of these transcription factors. We applied serial analysis of gene expression (SAGE) to identify targets of N-myc in neuroblastomas. Analysis of 42,000 mRNA transcript tags in SAGE libraries of N-myc- transfected and control neuroblastoma cells revealed 114 up-regulated genes. The majority of these genes have a role in ribosome assembly and activity. Northern blot analysis confirmed up-regulation of all tested transcripts. Induction was complete within 4 h after N-myc expression. The large majority of the ribosomal proteins were induced, as well as genes controlling rRNA maturation. Cellular rRNA content was 45% induced. SAGE libraries and northern blot analysis confirmed up-regulation of many of these genes in N-myc-amplified neuroblastomas. As N-myc can functionally replace c-myc, we analyzed whether N-myc targets were induced by c-myc as well. Approximately 40% of these N-myc targets were up-regulated in a c-myc-transfected melanoma cell line. These data suggest that myc genes function as major regulators of the protein synthesis machinery. |
| تدمد: | 1460-2075 0261-4189 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c431b994926510046e96fb28b9872fdc https://doi.org/10.1093/emboj/20.6.1383 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c431b994926510046e96fb28b9872fdc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602075 02614189 |
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