Inflammation Location, But Not Type, Determines the Increase in TGF-β1 and IGF-1 Expression and Collagen Deposition in IBD Intestine

التفاصيل البيبلوغرافية
العنوان: Inflammation Location, But Not Type, Determines the Increase in TGF-β1 and IGF-1 Expression and Collagen Deposition in IBD Intestine
المؤلفون: Lesley Maxwell, William F. Doe, Ian Craig Lawrance
المصدر: Inflammatory Bowel Disease. 7:16-26
بيانات النشر: Oxford University Press (OUP), 2001.
سنة النشر: 2001
مصطلحات موضوعية: Pathology, medicine.medical_specialty, Inflammation, In situ hybridization, Inflammatory bowel disease, Transforming Growth Factor beta1, Extracellular matrix, Collagen Type III, Transforming Growth Factor beta, Fibrosis, medicine, Humans, Immunology and Allergy, Insulin-Like Growth Factor I, Lamina propria, Chemistry, Gastroenterology, Inflammatory Bowel Diseases, medicine.disease, Immunohistochemistry, medicine.anatomical_structure, Gene Expression Regulation, Collagen, medicine.symptom, Transforming growth factor
الوصف: Background and Aims Inflammatory bowel disease (IBD) is frequently complicated by extracellular matrix (ECM) changes that may result in fibrosis. Transforming growth factor (TGF)-β1 and insulin-like growth factor (IGF)-1 mediate numerous ECM changes. Our aim was to determine whether TGF-β1 and IGF-1 are involved in intestinal ECM collagen regulation and what impact the inflammatory infiltrate has on their expression. Methods TGF-β1 and IGF-1 mRNA and protein were assessed in fibrosed Crohn's disease (CD), inflamed CD, inflamed ulcerative colitis (UC), and control intestine using in situ hybridization and immunohistochemistry. Collagen types I and III were quantified by electron immunohistochemistry. Results In CD, increased TGF-β1 and IGF-1 mRNA expression was transmural. In UC, the increase was confined to the lamina propria and submucosa. In both, distribution of TGF-β1 and IGF-1 protein matched mRNA expression and coincided with the distribution of the inflammatory infiltrate. An increase in the collagen type III:I ratio in both CD and UC also coincided with the inflammatory infiltrate. Conclusions These findings suggest that TGF-β1 and IGF-1 are involved in intestinal ECM remodeling in IBD, and their enhanced expression depends on the presence and location of inflammatory infiltrates rather than the type of IBD.
تدمد: 1078-0998
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c48503b37c4656b2974ee8d295e2cd99
https://doi.org/10.1097/00054725-200102000-00003
رقم الأكسشن: edsair.doi.dedup.....c48503b37c4656b2974ee8d295e2cd99
قاعدة البيانات: OpenAIRE