Ivermectin inhibits canine mammary tumor growth by regulating cell cycle progression and WNT signaling
| العنوان: | Ivermectin inhibits canine mammary tumor growth by regulating cell cycle progression and WNT signaling |
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| المؤلفون: | Haodi Dong, Degui Lin, Ying Zhao, Douglas H. Thamm, Hongxiu Diao, Huihao Xu, Di Zhang, Nan Cheng |
| المصدر: | BMC Veterinary Research, Vol 15, Iss 1, Pp 1-10 (2019) BMC Veterinary Research |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Cell cycle checkpoint, 040301 veterinary sciences, Cell Survival, β-Catenin, Mammary gland, Mice, Nude, Apoptosis, Mammary Neoplasms, Animal, Biology, 0403 veterinary science, 03 medical and health sciences, Mice, Ivermectin, Cyclin D1, Dogs, Cell Line, Tumor, parasitic diseases, medicine, Dog, Animals, Dog Diseases, beta Catenin, 030304 developmental biology, Cell Proliferation, Cancer, 0303 health sciences, Mammary tumor, lcsh:Veterinary medicine, General Veterinary, Antiparasitic Agents, Cell Cycle, Wnt signaling pathway, 04 agricultural and veterinary sciences, General Medicine, Neoplasms, Experimental, Gene Expression Regulation, Neoplastic, Wnt Proteins, medicine.anatomical_structure, Cell culture, Cancer research, lcsh:SF600-1100, Female, medicine.drug, Research Article |
| الوصف: | Background Mammary gland tumor is the most common spontaneous tumor in intact female dogs, and its poor prognosis remains a clinical challenge. Ivermectin, a well-known anti-parasitic agent, has been implicated as a potential anticancer agent in various types of human cancer. However, there are no reports evaluating the antitumor effects of ivermectin in canine mammary tumor. Here, we investigated whether ivermectin was able to inhibit canine mammary tumor development and explored the related mechanisms. Results Ivermectin inhibited the growth of canine mammary tumor cell lines in a dose- and time-dependent manner. The antitumor effects induced by ivermectin were associated with cell cycle arrest at G1 phase via down-regulation of CDK4 and cyclin D1 expression, with no significant induction of apoptosis. Furthermore, significantly reduced β-catenin nuclear translocation was observed after treatment with ivermectin, resulting in the inactivation of WNT signaling. Consistent with the results in vitro, a significant suppression of tumor growth by ivermectin was observed in canine mammary tumor xenografts. Conclusion Ivermectin, as a promising anti-cancer agent, inhibits the growth of canine mammary tumor by regulating cell cycle progression and WNT signaling. Electronic supplementary material The online version of this article (10.1186/s12917-019-2026-2) contains supplementary material, which is available to authorized users. |
| اللغة: | English |
| تدمد: | 1746-6148 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c593a12ba8f5b1c8f5b0376c7c075eec http://link.springer.com/article/10.1186/s12917-019-2026-2 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c593a12ba8f5b1c8f5b0376c7c075eec |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 17466148 |
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