The Actin-Based Motor Myosin Vb Is Crucial to Maintain Epidermal Barrier Integrity
| العنوان: | The Actin-Based Motor Myosin Vb Is Crucial to Maintain Epidermal Barrier Integrity |
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| المؤلفون: | Guy Serre, Marie Reynier, Alain Moga, Corinne Leprince, Sophie Allart, Dominique Goudounèche, Michel Simon |
| المساهمون: | Unité différenciation épidermique et auto-immunité rhumatoïde (UDEAR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Microscopie Électronique Appliquée à la Biologie (CMEAB), Hôpital de Rangueil, CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse]-Toulouse Réseau Imagerie-Genotoul ( TRI-Genotoul), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), SYNELVIA, Toulouse Réseau Imagerie-Genotoul ( TRI-Genotoul), CCSD, Accord Elsevier |
| المصدر: | Journal of Investigative Dermatology Journal of Investigative Dermatology, Nature Publishing Group, 2019, 139 (7), pp.1430-1438. ⟨10.1016/j.jid.2018.12.021⟩ Journal of Investigative Dermatology, 2019, 139 (7), pp.1430-1438. ⟨10.1016/j.jid.2018.12.021⟩ |
| بيانات النشر: | Elsevier BV, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Keratinocytes, 0301 basic medicine, Lamellar granule, Biochemistry, Cell junction, 0302 clinical medicine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Claudin-1, Myosin, MESH: Tight Junctions / metabolism, RNA, Small Interfering, MESH: Myosin Heavy Chains / genetics, Cytoskeleton, Cells, Cultured, MESH: Keratinocytes / metabolism, integumentary system, Chemistry, MESH: Epidermis / pathology, Cell biology, Actin Cytoskeleton, Protein Transport, MESH: Intercellular Signaling Peptides and Proteins / metabolism, medicine.anatomical_structure, 030220 oncology & carcinogenesis, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, symbols, Intercellular Signaling Peptides and Proteins, MESH: Myosin Heavy Chains / metabolism, Protein Binding, MESH: Cells, Cultured, MESH: Actin Cytoskeleton / metabolism, MESH: Protein Transport, Myosin Type V, Dermatology, MESH: Myosin Type V / genetics, Tight Junctions, MESH: Actins / metabolism, 03 medical and health sciences, symbols.namesake, MESH: Keratinocytes / pathology, medicine, Stratum corneum, Humans, MESH: Protein Binding, MESH: Claudin-1 / metabolism, Molecular Biology, Actin, MESH: Humans, Myosin Heavy Chains, MESH: rab GTP-Binding Proteins / metabolism, MESH: RNA, Small Interfering / genetics, MESH: Myosin Type V / metabolism, Cell Biology, Golgi apparatus, Actins, MESH: Epidermis / metabolism, 030104 developmental biology, rab GTP-Binding Proteins, Epidermis |
| الوصف: | International audience; Myosin Vb (Myo5b) is an unconventional myosin involved in the actin-dependent transport and tethering of intracellular organelles. In the epidermis, granular keratinocytes accumulate cytoplasmic lamellar bodies (LBs), secretory vesicles released at the junction with the stratum corneum that participate actively in the maintenance of the epidermal barrier. We have previously demonstrated that LB biogenesis is controlled by the Rab11a guanosine triphosphate hydrolase, known for its ability to recruit the Myo5b motor. In order to better characterize the molecular pathway that controls LB trafficking, we analyzed the role of F-actin and Myo5b in the epidermis. We demonstrated that LB distribution in granular keratinocytes was dependent on a dynamic F-actin cytoskeleton. Myo5b was shown to be highly expressed in granular keratinocytes and associated with corneodesmosin-loaded LB. In reconstructed human epidermis, Myo5b silencing led to epidermal barrier defects associated with structural alterations of the stratum corneum and a reduced pool of LB showing signs of disordered maturation. Myo5b depletion also disturbed the expression and distribution of both LB cargoes and junctional components, such as claudin-1, which demonstrates its action on both LB trafficking and junctional complex composition. Together, our data reveal the essential role of Myo5b in maintaining the epidermal barrier integrity. |
| وصف الملف: | application/pdf |
| تدمد: | 0022-202X 1523-1747 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5b23279e57f8e5cb9731c489548ae9e https://doi.org/10.1016/j.jid.2018.12.021 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c5b23279e57f8e5cb9731c489548ae9e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0022202X 15231747 |
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