Connexin 43-Mediated Mitochondrial Transfer of iPSC-MSCs Alleviates Asthma Inflammation
| العنوان: | Connexin 43-Mediated Mitochondrial Transfer of iPSC-MSCs Alleviates Asthma Inflammation |
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| المؤلفون: | Sinming Chiu, Shu-Bin Fang, Yuelin Zhang, Xin Li, Hung-Fat Tse, Zhi-Bin Xu, Qizhou Lian, Dan Jiang, Yin Yao, Xing-Liang Fan, Qing-Ling Fu |
| المصدر: | Stem Cell Reports, Vol 11, Iss 5, Pp 1120-1135 (2018) Stem Cell Reports |
| بيانات النشر: | Elsevier, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Ovalbumin, Induced Pluripotent Stem Cells, Connexin, Apoptosis, Inflammation, Lung injury, Biology, Mesenchymal Stem Cell Transplantation, Biochemistry, Article, connexin 43, Cell Line, Mice, 03 medical and health sciences, Genetics, medicine, Animals, Humans, Gene silencing, Induced pluripotent stem cell, Lung, lcsh:QH301-705.5, lcsh:R5-920, Nanotubes, Mesenchymal stem cell, mitochondrial transfer, Epithelial Cells, Mesenchymal Stem Cells, Cobalt, Cell Biology, asthma, Mitochondria, Cell biology, Transplantation, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, lcsh:Biology (General), iPSC-MSCs, Bone marrow, medicine.symptom, lcsh:Medicine (General), Developmental Biology |
| الوصف: | Summary We previously identified an immunomodulatory role of human induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (MSCs) in asthmatic inflammation. Mitochondrial transfer from bone marrow MSCs to epithelial cells can result in the attenuation of acute lung injury in mice. However, the effects of mitochondrial transfer from iPSC-MSCs to epithelial cells in asthma and the mechanisms underlying these effects are unclear. We found that iPSC-MSC transplantation significantly reduced T helper 2 cytokines, attenuated the mitochondrial dysfunction of epithelial cells, and alleviated asthma inflammation in mice. Tunneling nanotubes (TNTs) were formed between iPSC-MSCs and epithelial cells, and mitochondrial transfer from iPSC-MSCs to epithelial cells via TNTs was observed both in vitro and in mice. Overexpression or silencing of connexin 43 (CX43) in iPSC-MSCs demonstrated that CX43 plays a critical role in the regulation of TNT formation by mediating mitochondrial transfer between iPSC-MSCs and epithelial cells. This study provides a therapeutic strategy for targeting asthma inflammation. Graphical Abstract Highlights • iPSC-MSCs attenuate asthma inflammation and protect epithelial cells • The protection of iPSC-MSCs is attributable to mitochondrial transfer via TNTs • CX43-mediated TNT formation regulates the mitochondrial donation of iPSC-MSCs In this article, Fu, Lian, and colleagues show that mesenchymal stem cells derived from human induced pluripotent stem cells can transfer their mitochondria to rescue the injured bronchial epithelial cells, which attenuates asthma inflammation. Connexin 43, a component of gap junctions, plays a critical role during the process of mitochondrial transfer by regulating intercellular tunneling nanotube formation. |
| اللغة: | English |
| تدمد: | 2213-6711 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c63c46ced3c19dddff002689c952922f http://www.sciencedirect.com/science/article/pii/S2213671118303977 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c63c46ced3c19dddff002689c952922f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22136711 |
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