Paralog editing tunes rice stomatal density to maintain photosynthesis and improve drought tolerance
| العنوان: | Paralog editing tunes rice stomatal density to maintain photosynthesis and improve drought tolerance |
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| المؤلفون: | Nicholas G Karavolias, Dhruv Patel-Tupper, Kyungyong Seong, Michelle Tjahjadi, Gloria-Alexandra Gueorguieva, Jaclyn Tanaka, Ana Gallegos Cruz, Samantha Lieberman, Lillian Litvak, Douglas Dahlbeck, Myeong-Je Cho, Krishna K Niyogi, Brian J Staskawicz |
| المصدر: | Plant Physiology. 192:1168-1182 |
| بيانات النشر: | Oxford University Press (OUP), 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Physiology, Genetics, Plant Science |
| الوصف: | Rice (Oryza sativa) is of paramount importance for global nutrition, supplying at least 20% of global calories. However, water scarcity and increased drought severity are anticipated to reduce rice yields globally. We explored stomatal developmental genetics as a mechanism for improving drought resilience in rice while maintaining yield under climate stress. CRISPR/Cas9-mediated knockouts of the positive regulator of stomatal development STOMAGEN and its paralog EPIDERMAL PATTERNING FACTOR-LIKE10 (EPFL10) yielded lines with ∼25% and 80% of wild-type stomatal density, respectively. epfl10 lines with moderate reductions in stomatal density were able to conserve water to similar extents as stomagen lines but did not suffer from the concomitant reductions in stomatal conductance, carbon assimilation, or thermoregulation observed in stomagen knockouts. Moderate reductions in stomatal density achieved by editing EPFL10 present a climate-adaptive approach for safeguarding yield in rice. Editing the paralog of STOMAGEN in other species may provide a means for tuning stomatal density in agriculturally important crops beyond rice. |
| تدمد: | 1532-2548 0032-0889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c671a57774b7f55db3e6f7efb3c08da8 https://doi.org/10.1093/plphys/kiad183 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c671a57774b7f55db3e6f7efb3c08da8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
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