On the robustness of inference of association with the gut microbiota in stool, rectal swab and mucosal tissue samples
| العنوان: | On the robustness of inference of association with the gut microbiota in stool, rectal swab and mucosal tissue samples |
|---|---|
| المؤلفون: | Shan Sun, Qi Dai, Reid M. Ness, Anthony A. Fodor, M. Andrea Azcarate-Peril, Xiangzhu Zhu, Ivory C. Blakley, Martha J. Shrubsole, Alicia Sorgen, Xiang Huang, Douglas L. Seidner, Chang Yu, Harvey J. Murff |
| المصدر: | Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) Scientific Reports |
| بيانات النشر: | Nature Portfolio, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Male, medicine.drug_class, Science, Antibiotics, Physiology, Biology, Gut flora, Article, Body Mass Index, Feces, 03 medical and health sciences, Human health, Taxonomic composition, Sex Factors, Human gut, medicine, Humans, Intestinal Mucosa, Aged, 030304 developmental biology, Mucosal tissue, Aged, 80 and over, 0303 health sciences, Multidisciplinary, Bacteria, 030306 microbiology, Microbiota, Anti-Inflammatory Agents, Non-Steroidal, Age Factors, Rectum, Middle Aged, biology.organism_classification, Anti-Bacterial Agents, Gastrointestinal Microbiome, Metagenomics, Medicine, Female, Rectal swab, Microbiome |
| الوصف: | The gut microbiota plays an important role in human health and disease. Stool, rectal swab and rectal mucosal tissue samples have been used in individual studies to survey the microbial community but the consequences of using these different sample types are not completely understood. In this study, we report differences in stool, rectal swab and rectal mucosal tissue microbial communities with shotgun metagenome sequencing of 1397 stool, swab and mucosal tissue samples from 240 participants. The taxonomic composition of stool and swab samples was distinct, but less different to each other than mucosal tissue samples. Functional profile differences between stool and swab samples are smaller, but mucosal tissue samples remained distinct from the other two types. When the taxonomic and functional profiles were used for inference in association with host phenotypes of age, sex, body mass index (BMI), antibiotics or non-steroidal anti-inflammatory drugs (NSAIDs) use, hypothesis testing using either stool or rectal swab gave broadly significantly correlated results, but inference performed on mucosal tissue samples gave results that were generally less consistent with either stool or swab. Our study represents an important resource for determination of how inference can change for taxa and pathways depending on the choice of where to sample within the human gut. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c68f689198c58d3bbc35c8cedf19ea53 https://doaj.org/article/13d61f92d21f4c489d52b87400cedf98 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c68f689198c58d3bbc35c8cedf19ea53 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 20452322 |
|---|