Validation of a serum neutralization test for detection of antibodies specific to cyprinid herpesvirus 3 in infected common and koi carp (Cyprinus carpio)
| العنوان: | Validation of a serum neutralization test for detection of antibodies specific to cyprinid herpesvirus 3 in infected common and koi carp (Cyprinus carpio) |
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| المؤلفون: | S M Bergmann, G. Bovo, Thierry Morin, Lénaïg Louboutin, Marek Matras, J. Castric, Niels Jørgen Olesen, Joëlle Cabon, Olga Haenen |
| المصدر: | Journal of Fish Diseases, 40(5), 687-701 Journal of Fish Diseases 40 (2017) 5 |
| بيانات النشر: | Wiley, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Carps, Epidemiology, Bioinformatica & Diermodellen, Veterinary (miscellaneous), Cyprinid herpesvirus 3, ved/biology.organism_classification_rank.species, Aquatic Science, Antibodies, Viral, Neutralization, Cyprinus, Fish Diseases, 03 medical and health sciences, Common carp, Neutralization Tests, Bio-informatics & Animal models, Animals, Epidemiology, Bio-informatics & Animal models, Carp, Herpesviridae, Subclinical infection, Epidemiologie, biology, ved/biology, method validation, Herpesviridae Infections, biology.organism_classification, Virology, serum neutralization test, 030104 developmental biology, CyHV-3-specific antibodies, Viral replication, Epidemiologie, Bioinformatica & Diermodellen, common carp, biology.protein, koi, Antibody |
| الوصف: | Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of a serious infective, notifiable disease affecting common carp and varieties. In survivors, infection is generally characterized by a subclinical latency phase with restricted viral replication. The CyHV-3 genome is difficult to detect in such carrier fish that represent a potential source of dissemination if viral reactivation occurs. In this study, the analytical and diagnostic performance of an alternative serum neutralization (SN) method based on the detection of CyHV-3-specific antibodies was assessed using 151 serum or plasma samples from healthy and naturally or experimentally CyHV-3-infected carp. French CyHV-3 isolate 07/108b was neutralized efficiently by sera from carp infected with European, American and Taiwanese CyHV-3 isolates, but no neutralization was observed using sera specific to other aquatic herpesviruses. Diagnostic sensitivity, diagnostic specificity and repeatability of 95.9%, 99.0% and 99.3%, respectively, were obtained, as well as a compliance rate of 89.9% in reproducibility testing. Neutralizing antibodies were steadily detected in infected carp subjected to restrictive or permissive temperature variations over more than 25 months post-infection. The results suggest that this non-lethal diagnostic test could be used in the future to improve the epidemiological surveillance and control of CyHV-3 disease. |
| وصف الملف: | application/octet-stream; application/pdf |
| تدمد: | 0140-7775 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6c6bae8312af256e68d2582a5791417 https://doi.org/10.1111/jfd.12550 |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....c6c6bae8312af256e68d2582a5791417 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01407775 |
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