Comparing activated sludge fungal community population diversity using denaturing gradient gel electrophoresis and terminal restriction fragment length polymorphism

التفاصيل البيبلوغرافية
العنوان: Comparing activated sludge fungal community population diversity using denaturing gradient gel electrophoresis and terminal restriction fragment length polymorphism
المؤلفون: Robert J. Seviour, Garth O. Watson, Tegan N. Evans, Gavin N. Rees
بيانات النشر: La Trobe, 2023.
سنة النشر: 2023
مصطلحات موضوعية: Genetics, Phylogenetic tree, Sewage, Denaturing Gradient Gel Electrophoresis, Fungi, food and beverages, General Medicine, Biodiversity, Ribosomal RNA, Biology, Microbiology, Molecular biology, 18S ribosomal RNA, Terminal restriction fragment length polymorphism, chemistry.chemical_compound, Activated sludge, chemistry, DNA, Ribosomal Spacer, RNA, Ribosomal, 18S, Molecular Biology, Gene, DNA, Temperature gradient gel electrophoresis, Polymorphism, Restriction Fragment Length
الوصف: MDFRC item.We compared the relative values of denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) for profiling fungal communities in wastewater treatment plants using both ITS and 18S rRNA gene fragments as phylogenetic markers. A similar number of fungal ribotypes was obtained with both methods for the same treatment plant when the ITS primer set was used, while a greater number of ribotypes was obtained with T-RFLP compared to DGGE with the 18S rRNA primer set. Non-metric multi-dimensional scaling of presence/absence data and analysis of similarity showed that both methods could distinguish between the different plant communities at a statistically significant level (p < 0.05), regardless of which phylogenetic marker was used. The data suggest that both methods can be used preferably together to profile activated sludge fungal communities. A comparison of profiles generated with both these phylogenetic markers based on the number of ribotypes/bands, suggests that the 18S rRNA region is more discriminatory than the ITS region. Detected differences in fungal community compositions between plants probably reflect differences in their influent compositions and operational parameters.
DOI: 10.26181/22276624.v1
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c75170b5e324604122ee0392bd066738
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....c75170b5e324604122ee0392bd066738
قاعدة البيانات: OpenAIRE