Lipid metabolism of leukocytes in the unstimulated and activated states
| العنوان: | Lipid metabolism of leukocytes in the unstimulated and activated states |
|---|---|
| المؤلفون: | Johannes H. von Hegedus, Alejandro Ondo-Méndez, Hilde Brouwers, Evelyne Steenvoorden, Oleg A. Mayboroda, Andreea Ioan-Facsinay, Juan Carlos Alarcon-Barrera, Martin Giera |
| المصدر: | Analytical and Bioanalytical Chemistry, 412(10), 2353-2363. SPRINGER HEIDELBERG Analytical and Bioanalytical Chemistry Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Comprehensive analysis, CD14+, Neutrophils, CD14, Stimulation, Phosphatidyl choline, Lipidyzer (TM), 01 natural sciences, Biochemistry, Mass Spectrometry, Analytical Chemistry, CD4+, 03 medical and health sciences, Differential mobility spectrometries, Cell Line, Tumor, Lipidomics, Leukocytes, Humans, Lipidyzer™, Phospholipids, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Chemistry, T-cells, 010401 analytical chemistry, Fatty acid, Lipid metabolism, IPA extraction, Metabolism, Lipidome, Lipid Metabolism, Lipids, 0104 chemical sciences, Analytical technology, Correlation coefficient, Fatty Acids, Unsaturated, Polyunsaturated fatty acids, Cytology, Well stimulation, Research Paper, Polyunsaturated fatty acid |
| الوصف: | Lipidomics has emerged as a powerful technique to study cellular lipid metabolism. As the lipidome contains numerous isomeric and isobaric species resulting in a significant overlap between different lipid classes, cutting-edge analytical technology is necessary for a comprehensive analysis of lipid metabolism. Just recently, differential mobility spectrometry (DMS) has evolved as such a technology, helping to overcome several analytical challenges. We here set out to apply DMS and the Lipidyzer™ platform to obtain a comprehensive overview of leukocyte-related lipid metabolism in the resting and activated states. First, we tested the linearity and repeatability of the platform by using HL60 cells. We obtained good linearities for most of the thirteen analyzed lipid classes (correlation coefficient > 0.95), and good repeatability (%CV |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c79aa4d016e0d720ef1c7aabb72028c8 https://link.springer.com/article/10.1007/s00216-020-02460-8 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c79aa4d016e0d720ef1c7aabb72028c8 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |