The Effect of Tong-Xie-Yao-Fang on Intestinal Mucosal Mast Cells in Postinfectious Irritable Bowel Syndrome Rats
| العنوان: | The Effect of Tong-Xie-Yao-Fang on Intestinal Mucosal Mast Cells in Postinfectious Irritable Bowel Syndrome Rats |
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| المؤلفون: | Lin Lv, Xiangxue Ma, Fengyun Wang, Hai-Jie Ji, Yuan-Zhi Duan, Ya-Xin Tian, Zheng Rui, Ting Chen, Xiao-Ge Wang, Nan Kang, Xiao-Lan Yin, Xudong Tang |
| المصدر: | Evidence-Based Complementary and Alternative Medicine, Vol 2017 (2017) Evidence-based Complementary and Alternative Medicine : eCAM |
| بيانات النشر: | Hindawi Limited, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | medicine.medical_specialty, Article Subject, Withdrawal reflex, Tryptase, Immunofluorescence, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Intestinal mucosa, Internal medicine, medicine, Irritable bowel syndrome, biology, medicine.diagnostic_test, lcsh:Other systems of medicine, lcsh:RZ201-999, Mast cell, medicine.disease, Endocrinology, medicine.anatomical_structure, Complementary and alternative medicine, chemistry, 030220 oncology & carcinogenesis, Hyperalgesia, biology.protein, 030211 gastroenterology & hepatology, medicine.symptom, Histamine, Research Article |
| الوصف: | Objective. To investigate the effects of Tong-Xie-Yao-Fang (TXYF) on intestinal mucosal mast cells in rats with postinfectious irritable bowel syndrome (PI-IBS).Design. PI-IBS rat models were established using a multistimulation paradigm. Then, rats were treated with TXYF intragastrically at doses of 2.5, 5.0, and 10.0 g·kg−1·d−1for 14 days, respectively. Intestinal sensitivity was assessed based on abdominal withdrawal reflex (AWR) scores and fecal water content (FWC). Mast cell counts and the immunofluorescence of tryptase and c-Fos in intestinal mucosa were measured; and serum IL-1β, TNF-α, and histamine levels were determined.Results. AWR reactivity and FWC which were significantly increased could be observed in PI-IBS rats. Remarkably increased mast cell activation ratio in intestinal mucosa, together with increased serum TNF-αand histamine levels, could also be seen in PI-IBS rats; furthermore, PI-IBS-induced changes in mast cell activation and level of serum TNF-αand histamine could be reversed by TXYF treatment. Meanwhile, tryptase and c-Fos expression were also downregulated.Conclusion. TXYF improves PI-IBS symptoms by alleviating behavioral hyperalgesia and antidiarrhea, the underlying mechanism of which involves the inhibitory effects of TXYF on activating mucosal mast cells, downregulating tryptase and c-Fos expression, and reducing serum TNF-αand histamine levels. |
| وصف الملف: | text/xhtml |
| تدمد: | 1741-4288 1741-427X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7e35074aa00920e6a8f841a5cb02b30 https://doi.org/10.1155/2017/9086034 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c7e35074aa00920e6a8f841a5cb02b30 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17414288 1741427X |
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