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A multispecies outbreak of carbapenem-resistant bacteria harboring the blaKPC gene in a non-classical transposon element

التفاصيل البيبلوغرافية
العنوان: A multispecies outbreak of carbapenem-resistant bacteria harboring the blaKPC gene in a non-classical transposon element
المؤلفون: Jose M. Munita, Piero Guggiana, Cristian Figueroa, Lina M Rivas, Aniela Wozniak, Claudia Castillo, Francisco Moya-Flores, Patricia García
المصدر: BMC Microbiology
BMC Microbiology, Vol 21, Iss 1, Pp 1-10 (2021)
بيانات النشر: BioMed Central, 2021.
سنة النشر: 2021
مصطلحات موضوعية: Microbiology (medical), Transposable element, Gene Transfer, Horizontal, Klebsiella pneumoniae, KPC Carbapenemase, lcsh:QR1-502, Multispecies outbreak, medicine.disease_cause, Microbiology, lcsh:Microbiology, beta-Lactamases, Disease Outbreaks, 03 medical and health sciences, Plasmid, Carbapenem resistant Enterobacteriaceae, medicine, polycyclic compounds, Humans, Insertion sequence, 030304 developmental biology, 0303 health sciences, biology, Bacteria, 030306 microbiology, Pseudomonas aeruginosa, Genetic transfer, Horizontal gene transfer, Bacterial Infections, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, bacterial infections and mycoses, Citrobacter freundii, Carbapenem-Resistant Enterobacteriaceae, Carbapenems, DNA Transposable Elements, Enterobacter cloacae, Research Article
الوصف: Background Klebsiella pneumoniae is the most frequent KPC-producing bacteria. The blaKPC gene is frequently embedded in Tn4401 transposon, and less frequently in non-Tn4401 elements (NTEKPC) variants I-III. The first case of KPC in the UC-CHRISTUS Clinical Hospital was detected in Pseudomonas aeruginosa. Soon after this event, KPC was detected in 2 additional Pseudomonas aeruginosa, 3 Escherichia coli, 3 Enterobacter cloacae, 3 Klebsiella pneumoniae, and 1 Citrobacter freundii, isolated from 6 different patients. We aimed to elucidate the possible mechanisms of genetic transfer and dissemination of the blaKPC gene among isolates of this multispecies outbreak. A molecular epidemiology analysis of the above mentioned clinical isolates (n = 13) through Multi-Locus Sequence Typing, plasmid analysis, Pulsed-Field Gel-Electrophoresis, and Whole-genome sequencing (WGS) was performed. Results High-risk sequence types were found: K. pneumoniae ST11, P. aeruginosa ST654, and E. cloacae ST114. All enterobacterial isolates were not clonal except for 3 E. coli isolated from the same patient. WGS analysis in 6 enterobacterial isolates showed that 4 of them had blaKPC embedded in a novel variant of NTEKPC designated NTEKPC-IIe. Upstream of blaKPC gene there was a 570 pb truncated blaTEM-1 gene followed by an insertion sequence that was 84% similar to ISEc63, a 4473 bp element of the Tn3 family. Downstream the blaKPC gene there was a truncated ISKpn6 gene, and the inverted repeat right sequence of Tn4401. The ISec63-like element together with the blaKPC gene plus Tn4401 remnants were inserted in the Tra operon involved in conjugative transfer of the plasmid. This NTE was carried in a broad host-range IncN plasmid. P. aeruginosa isolates carried blaKPC gene embedded in a typical Tn4401b transposon in a different plasmid, suggesting that there was no plasmid transfer between Enterobacteriaceae and P. aeruginosa as initially hypothesized. Conclusions Most enterobacterial isolates had blaKPC embedded in the same NTEKPC-IIe element, suggesting that this multispecies KPC outbreak was due to horizontal gene transfer rather than clonal spread. This poses a greater challenge to infection control measures often directed against containment of clonal spread.
اللغة: English
تدمد: 1471-2180
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8246b9c68d7ab7b7f6a15951c992394
http://europepmc.org/articles/PMC8034096
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....c8246b9c68d7ab7b7f6a15951c992394
قاعدة البيانات: OpenAIRE
الوصف
تدمد:14712180