Cytotoxic and cytostatic effects of four Annonaceae plants on human cancer cell lines
| العنوان: | Cytotoxic and cytostatic effects of four Annonaceae plants on human cancer cell lines |
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| المؤلفون: | Jiraprapa Wipasa, Chalermpong Saenjum, Kriangkrai Chawansuntati, Tanawat Chaowasku, Boonsong Wungsintaweekul, Maslin Osathanunkul, Pathrapol Lithanatudom, Kitti Pumiputavon |
| المصدر: | In Vitro Cellular & Developmental Biology - Animal. 55:723-732 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Cell cycle checkpoint, Annonaceae, Antineoplastic Agents, Apoptosis, Biology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Cell Line, Tumor, Neoplasms, Humans, Cytotoxic T cell, Propidium iodide, Cell Proliferation, Traditional medicine, Plant Extracts, Cell Cycle Checkpoints, Hep G2 Cells, Cell Biology, General Medicine, Cell cycle, biology.organism_classification, Plant Leaves, 030104 developmental biology, chemistry, Phytochemical, 030220 oncology & carcinogenesis, Bullatacin, Developmental Biology |
| الوصف: | Several species of the Annonaceae plants have been used as complementary medicine for cancer-associated illnesses in some ethnic groups of northern Thailand. This study investigated the cytotoxic and cytostatic activity of methanolic extracts derived from the stems of these plants, including Uvaria longipes (Craib) L.L.Zhou, Y.C.F.Su & R.M.K.Saunders, Artabotrys burmanicus A.DC, Marsypopetalum modestum (Pierre) B.Xue & R.M.K.Saunders, and Dasymaschalon sp. Cell death induction of seven human cancer cell lines and cell cycle analyses were assessed by Annexin V and/or propidium iodide (PI) staining and analyzed by flow cytometry. Treatment of cancer cell lines with the extract of four Annonaceae plants resulted in various cytotoxic activities depending on cell type. The extract of U. longipes exhibited the highest cytotoxic activity capable of inducing cell death of several cancer cell lines, particularly against hepatocellular carcinoma cell lines (HepG2 and Hep3B). This extract was capable of inducing cell cycle arrest at the SubG1 phase. Phytochemical screening of all the extracts revealed the presence of alkaloids, sterols, tannins, anthraquinone glycoside, coumarin, and flavonoids. Determination of active compounds by high-performance liquid chromatography standards revealed bullatacin and asiminecin in all the extracts. The extract of Annonaceae stem or its compounds may provide an opportunity for the development of new therapies against cancer. |
| تدمد: | 1543-706X 1071-2690 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8dd5b16c1f8544e3d4197a02da6f3d5 https://doi.org/10.1007/s11626-019-00393-w |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....c8dd5b16c1f8544e3d4197a02da6f3d5 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 1543706X 10712690 |
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