Nonlinear scanning structured illumination microscopy based on nonsinusoidal modulation
العنوان: | Nonlinear scanning structured illumination microscopy based on nonsinusoidal modulation |
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المؤلفون: | Lei Wang, Bruce Z. Gao, Yonghong Shao, Xiaomin Zheng, Youjun Zeng, Wang Meiting, Jie Zhou, Junle Qu, Jiajie Chen |
المصدر: | Journal of Innovative Optical Health Sciences, Vol 14, Iss 5, Pp 2142002-1-2142002-8 (2021) |
بيانات النشر: | World Scientific Publishing, 2021. |
سنة النشر: | 2021 |
مصطلحات موضوعية: | Technology, Materials science, business.industry, Resolution (electron density), Biomedical Engineering, Structured illumination microscopy, Medicine (miscellaneous), structured illumination microscopy, QC350-467, Optics. Light, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials, Nonlinear system, Optics, Modulation, super-resolution image, Microscopy, nonsinusoidal function, business |
الوصف: | Structured illumination microscopy (SIM) is an essential super-resolution microscopy technique that enhances resolution. Several images are required to reconstruct a super-resolution image. However, linear SIM resolution enhancement can only increase the spatial resolution of microscopy by a factor of two at most because the frequency of the structured illumination pattern is limited by the cutoff frequency of the excitation point spread function. The frequency of the pattern generated by the nonlinear response in samples is not limited; therefore, nonlinear SIM (NL-SIM), in theory, has no inherent limit to the resolution. In the present study, we describe a two-photon nonlinear SIM (2P-SIM) technique using a multiple harmonics scanning pattern that employs a composite structured illumination pattern, which can produce a higher order harmonic pattern based on the fluorescence nonlinear response in a 2P process. The theoretical models of super-resolution imaging were established through our simulation, which describes the working mechanism of the multi-frequency structure of the nonsinusoidal function to improve the resolution. The simulation results predict that a 5-fold improvement in resolution in the 2P-SIM is possible. |
اللغة: | English |
تدمد: | 1793-7205 1793-5458 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8f5bd17d5521cc3744b07625b307504 http://www.worldscientific.com/doi/epdf/10.1142/S1793545821420025 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....c8f5bd17d5521cc3744b07625b307504 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 17937205 17935458 |
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